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Accueil du site → Doctorat → Mexique → Determinación del nivel de tolerancia a salinidad de Paulownia imperialis (Siebold & Zuccarini) y Paulownia fortunei (Seemann & Hemsley) aplicando cloruro de sodio en condiciones in vitro

Centro de Investigaciones Biológicas del Noroeste (CIBNOR) 2008

Determinación del nivel de tolerancia a salinidad de Paulownia imperialis (Siebold & Zuccarini) y Paulownia fortunei (Seemann & Hemsley) aplicando cloruro de sodio en condiciones in vitro

Ayala Astorga, Gloria Irma

Titre : Determinación del nivel de tolerancia a salinidad de Paulownia imperialis (Siebold & Zuccarini) y Paulownia fortunei (Seemann & Hemsley) aplicando cloruro de sodio en condiciones in vitro

Auteur : Ayala Astorga, Gloria Irma

Etablissement de soutenance : Centro de Investigaciones Biológicas del Noroeste (CIBNOR)

Grade : Doctorado en Ciencias en el Uso, Manejo y Preservación de los Recursos Naturales 2008

Résumé
This study deals with tissue culture techniques that were developed to propagate Paulownia imperialis and Paulownia fortunei and to conduct experiments. Seeds of both species were germinated in WPM culture medium. Once the seedlings developed to a standar size, two to three months old, they were transferred to a culture medium containing sodium chloride in different concentrations. Biochemical, physiological and structurals analysis were performed in two periods of time to know the effects of saline stress on both Paulownia species. Initially we tested WPM culture media with sodium chloride at concentrations : 0, 50, 75, 100, 200, 300, 400, 500 and 1000 mM. The results on the effects of salinity by sodium chloride in Paulownia imperialis and Paulownia fortunei in vitro showed that sodium chloride concentrations adversely affected the growth and development of plants because the plants of both species died after two weeks in the culture medium with higher levels of sodium chloride. We tested other WPM culture medium WPM with different levels of sodium chloride with : 0, 20, 40, 60, 80 and 160 mM and performed determinations at 15 and 30 days of fresh weight in leaves, stems and roots, dry weight in leaves, stems and roots, water content in leaves, stems and roots, leaf area, observations with the scanning electron microscope, pigments, ionic composition such as Na+, K+, Ca++ and Mg++ in leaves, stems and roots, relationship K+/Na+ and TBARS, determination of protein and peroxidases, catalase, and proline content at 30 days and determinations of photosynthesis, respiration, stomata conductance, WUE and Ci/Ca in 19 and 26 days. The results show that the sodium has toxic effects on both species of Paulownia, causing an imbalance of nutrients by reducing the fresh weight and dry weight as well as leaf area, and also decreased chlorophyll a and chlorophyll b, -carotene and violaxanthin. In addition there was an imbalance in metabolic functions with the resulting disruption in the physiological reactions of plants to affect proline and proteins content, enzyme activity as well as photosynthesis. The results of this study indicate that both species can be considered moderately tolerant to stress caused by sodium chloride although observed better adaptation of Paulownia imperialis in the moderate salinity conditions

Mots Clés : Cultivo de tejidos ; tolerancia ; salinidad ; Paulownia imperialis ; Paulownia fortunei ; cloruro de sodio ;

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Page publiée le 29 septembre 2017