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Accueil du site → Master → Etats Unis → 1979 → Characterization and identification of an isolate of halobacterium from Soda Springs Mojave Desert

California State University - San Bernardino (1979)

Characterization and identification of an isolate of halobacterium from Soda Springs Mojave Desert

Abayomi Odubela

Titre : Characterization and identification of an isolate of halobacterium from Soda Springs Mojave Desert

Auteur : Abayomi Odubela

Université de soutenance : California State University - San Bernardino

Grade : Master of Science in Biology 1979

Résumé
A strain of Halobacterium . isolated from a pond at the California State Universities and Colleges Desert . Studies Center |at , Soda Springs, Mojave Desert, California „ was studied in order to define its taxonomic place in the genus Halohactelrium. To define the characteristics, of this ’bacterium, ’ , standard physiological activities, as listed in Bergey’s Manual of Determinative Bacteriology, 8^ Ed. (Gibbons 197^) were studied, Additionally, the red pigment t produced, by ; the organism was characterized, , and the, molar percent guanine plus cytosine determined. The organism is aerobic, non-motile, Gram*", and pleomorphic, exhibiting both swollen rods and coccoid .cell form. It grows well at a temperature between 32 to 33°C, and requires at :, least 18 % NaCl for growth, v Optimuxn occurs at a NaCl ooncentration between 25 and. 30%. It does not liquefy gelatin, indole is not produced and the organism does not. reduce nitrate, nor produce nitrogen. There is no unequivocable evidence that the’ organism ,utiIizes . .. carbohydrates, The red pigment in the organism is ■ ; bacterioruberin, as supported, by spectral and TLG mobility data. The DNA Of the organism consists of two components having’59 and 6|8 molar percent guanine plus cytosine : respectively,. The organism is, classified as a strain, of Halobacterium salinarium, on the basis of the various characteristics indicated. above

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