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Jordan University of Science and Technology (JUST) 2014

Antimicrobial Activity of Theree Selected Jordanian Medicinal Plant Extracts Against Several Pathogenic Bacterial Types

Wala muhammad abdala alassuly

Titre : Antimicrobial Activity of Theree Selected Jordanian Medicinal Plant Extracts Against Several Pathogenic Bacterial Types

Auteur : Wala muhammad abdala alassuly

Université de soutenance : Jordan University of Science and Technology (JUST)

Grade : Master of Science (MS) 2014

Résumé
At the present time, drug resistance in pathogenic bacteria is a major and serious problem. Therefore, plant origin herbal medicines are considered safe and may be effective alternatives to synthetic drugs. The antimicrobial effects of many herbs and spices have been recognized for centuries by many cultures. In Jordan a large number of plant species are reported to have medicinal properties. Some of these plants are commonly used and have been used by people as folk medicine for hundreds of years. In this study we focused on three commonly used Jordanian medicinal plants that have been shown to be effective against certain human pathogens and they are : Peganum harmala L, Pistacia palaestina Boiss and Brassica oleracea L.
Goals : This study is aimed to determine the antimicrobial activity of three medicinal plants against a group of pathogenic gram positive and gram negative bacteria (Escherichia coli, Staphylococcus aureus (MRSA ), Pseudomonas aeruginosa, Klebseilla pneumonia, Acinetobacter baumannii, Proteus mirabilis, Neisseria meningitides, Haemophilus influenza and Helicobacter pylori).
Methods : Ethanolic extracts of P.harmala (aerials and seeds), P. palaestina (fruits) and B.oleracea (seeds) were tested separately against the growth of the selected bacterial pathogens. Three different concentrations (50 mg/ml, 100 mg/ml and 300 mg/ml) were prepared from each extract and the crude extracts were subjected to further fractionation. Extraction of B.oleracea (seeds) was achieved using different solvents that included : 95% ethanol, 60 methanol, acetone, ethyl acetate, water and chloroform. Disk diffusion method and agar well diffusion method were used to determine the antimicrobial activity of each extract of different parts of each plant. To evaluate the Minimum Inhibitory Concentration (MIC) of these plants’ antibacterial activities against selected bacterial species, the antimicrobial activity of butanol extracts of these plant species was determined using a deep-well broth microdilution method on tested bacterial strains.
Results : The ethanol fraction of P.harmala (aerials) showed weak activity against all bacterial strains tested, while the butanol fraction of P.harmala (seeds) and P. palaestina (fruits) were the most effective fractions against the tested bacterial strains. While, 60% methanol extract of B.oleracea (seeds) showed the highest activity. Minimal inhibitory concentration value for P.harmala (seeds) butanol fraction against S.aureus was 0.156 (mg/ml), A.baumannii 0.625 (mg/ml), E.coli 0.312 (mg/ml), K. pneumonia 0.312 (mg/ml), P.aeruginosa 0.625 (mg/ml), and P.mirabilis 0.156 (mg/ml). Meanwhile, Minimal inhibitory concentration value for and P.palaestina (fruits) butanol fraction against S.aureus was 0.625 (mg/ml), A.baumannii 1.25 (mg/ml), E.coli 2.5 (mg/ ml), K. pneumonia 2.5 (mg/ ml), P.aeruginosa 2.5 (mg / ml), and P.mirabilis 1.25 (mg/ml).
Conclusion : The antibacterial activity of Peganum harmala, Pistacia palaestina and Brassica oleracea extract against the different bacterial species strengthen the scientific evidence of the effectiveness of medicinal plants in the treatment of infections which makes these plants potential candidates for new drugs.

Mots clés : Peganum harmala L, Pistacia palaestina Boiss and Brassica oleracea L

Présentation

Page publiée le 20 mars 2018