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Martin-Luther-Universität Halle-Wittenberg (2017)

Association genetics of biomass development under drought stress using high-throughput phenotyping in barley (Hordeum vulgare L.)

Dhanagond, Sidram

Titre : Association genetics of biomass development under drought stress using high-throughput phenotyping in barley (Hordeum vulgare L.)

Auteur : Dhanagond, Sidram

Université de soutenance : Martin-Luther-Universität Halle-Wittenberg

Grade : Doctoral thesis 2017

Résumé
A diverse set of 100 two-rowed spring barley accessions was phenotyped for biomass development under seasonal drought stress in three consecutive experiments, on an imaging-based high throughput phenotyping (HTP) platform. Drought stress was imposed to mimic seasonal drought occurring during vegetative phase of development. The setup included a recovery period after re-watering. Results show that biomass development is a dynamic process and is affected by drought stress, significantly reducing the final biomass. Genome-wide association studies (GWAS), utilizing 9k iSelect SNP data, showed that different QTLs influence biomass at different developmental stages. In total, 13 biomass QTL were detected ; most of them specific either for the drought or the recovery phase. Five biomass QTL co-localized with known flowering time genes, showing the impact of phenology on biomass. In another set of greenhouse experiments, a subset of 26 accessions was evaluated until maturity to estimate the effect of biomass and seasonal drought stress on final yield traits. Drought negatively affected most the grain yield. Further, a positive correlation was observed between vegetative biomass and grain yield in well-watered and seasonal drought conditions

Mots clés  : Barley ; biomass ; drought stress ; quantitative trait loci (QTL) ; dynamic QTL ; genome-wide association studies (GWAS) ; high-throughput phenotyping ; recovery

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Page publiée le 23 septembre 2017, mise à jour le 15 mai 2018