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Université de Zhejiang (2015)

Specific Proteins and Relevant Genes Associated with Drought Tolerance in Tibetan Wild Barley and Alleviation Effects of Exogenous Betaine on Drought Stress

王南博;

Titre : Specific Proteins and Relevant Genes Associated with Drought Tolerance in Tibetan Wild Barley and Alleviation Effects of Exogenous Betaine on Drought Stress

Auteur : 王南博;

Grade : Doctoral Dissertation 2015

Université : Université de Zhejiang

Résumé partiel
Drought is one of major meteorological disaster constraining crop productivity worldwide. One of the cost-effective and efficient approaches is to develop drought resistant/tolerant crop cultivars to ensure agricultural production. To breed crop cultivars tolerant to drought stress, it is important to identify genetic resources in potential of drought tolerance and understand its mechanism. Tibetan annual wild barley (H. vulgare ssp. spontaneum and ssp.agriocrithum) posses rich in genetic diversity and specific genes associated with abiotic-tolerance for the improvement of modern barley cultivars. The present study was carried out, using drought-tolerant Tibetan wild barley genotype (XZ5) identified in our previous research, to identify stress-specific proteins for drought tolerance in wild barley via two-dimensional gel electrophoresis and mass spectrometry and bio-informatics. According to the data of proteomic analyses, coding sequences (CDS) of drought tolerance relevant genes were cloned from different wild barley geneotypes (XZ5and drought-sensitive XZ54) and cv. ZAU3by degenerate PCR, followed by databases homologous searches. Meanwhile, we investigated the protective effect of foliar-application of betaine against drought-induced growth and photosynthesis inhibition, oxidative stress and damage in ultrastructure in barley seedlings. The main results were summarized as follows:1. Comparative proteomic analysis of drought tolerance in the two contrasting Tibetan wild barley genotypes and cv ZAU3sA greenhouse pot experiment was performed to compare proteomic characteristics of two contrasting Tibetan wild barley genotypes (drought-tolerant XZ5and drought-sensitive XZ54) and cv. ZAU3under drought stress at soil moisture content10%(SMC10) and4%(SMC4) and subsequently2days (R1) and5days (R2) of recovery. More than1700protein spots were identified in each gel. We identified38drought-tolerance-associated proteins, including Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit (rbcL) and Thioredoxin M (Trx-M), through mass spectrometry and data bank analysis. These proteins were mainly categorized into photosynthesis, stress response, metabolic process, energy and amino-acid biosynthesis. Specifically,6protein spots were exclusively expressed or up-regulated under drought stress in XZ5but not in XZ54, including melanoma-associated antigen p97, type I chlorophyll a/b-binding protein b, glutathione S-transferase1, ribulosebisphosphate carboxylase large chain. Moreover, type I chlorophyll a/b-binding protein b was specifically expressed in XZ5but not in either XZ54or ZAU3.

Mots clés : Drought stress; Barley (Hordeum vulgare L.); Tibetan wild barley; Massspectrometry; Gene clone; Glycine betaine; ROS metabolism; Specific protein; Ultrastructure

Présentation (CNKI)

Page publiée le 4 octobre 2017