Informations et ressources scientifiques
sur le développement des zones arides et semi-arides

Titre : Molecular and Protein Analysis for the Identification of Salt Tolerance of the In-vitro Propagated Lemon Genotypes

التحليل الجزيئي والبروتيني لتحمل الملوحة في اصناف الليمون الناتجة من الاكثار المعمليالدقيق

Auteur : Al Sayed, AbdulMohsin Radah

Université de soutenance : King Abdulaziz University

Grade : Doctor 2017

Résumé partiel
The presented study considered the in vitro propagation using nodal segments and DNA fingerprinting of four lemon culttivars. The experiments were conducted during 2012-2014 in the Lab of Plant Tissue Culture and the Lab of Plant Biotechnology, Department of Arid Land Agriculture, Faculty of Meteorology, Environment and Arid Land Agriculture, King Abdulaziz University. The first experiment considered the effects of growth regulators on in vitro nodal segments of the lemon cultivars. The second experiment investigated the impacts of adding different growth regulators combinations at low levels to the culture medium for enhancing shoots multiplications and elongations. The third experiment investigated root induction and roots formation of the lemon formed plantlets. The fourth experiment focused on in vitro secreening of lemon cultivars to salinity tolerance. The fifth experiment focused on the DNA fingerprinting and possibly genetic diversity of the four tested lemon cultivars using RAPD-based PCR technique. The experiments of in vitro culture of Lemon were laid out in Completely Randomized Design (CRD) using 4 replicates. The results of first experiment revealed obvious significant differences due to effects of lemon cultivars, plant growth regulators combinations and their interactions on in vitro nodal segments of lemon at all measured traits. The results illustrated that the low concentrations of Kin and BAP alone or in combination with 2,4-D caused early buds sprouting of all lemon cultivars. The lemon cultivars ‘Shehri’ and ‘Aln-Eureka’ revealed highest percentages of sprouted buds on culture medium supplemented with a combinations of BAP at high concentrations and 2,4-D at low concentrations. It was observed no dead shootlets of ‘Shaary’, ‘Aln-Eurka’ and ‘Banziher’on culture medium contained low concentrations of Kin, BAP alone or in combinations with 2,4-D. The tallest primary shoots were produced by ‘Shehri’ (25.4mm) on cultutre medium contained BAP at 0.5 mgl-1+2,4-D at 1mgl-1. The lemon cultivar ‘Aln-Eureka’ produced the maxiumum number of leaves/shoot on culture medium contained BAP 0.5mgl-1 + 2,4-D at 1mgl-1. For shoot multiplication, the results showed that all explants of ‘Aln-Eureka’ developed shoots on culture medium supplemented with Kin 1mgl-1 and BAP at 0.5mgl-1 and 1mgl-1. Adding BAP at 0.5mgl-1 and 1mgl-1 to the culture medium enhanced shoots formation of the lemon cultivar ‘Aln-Eureka’ and ‘Shaary’. The greatest lengths of formed shoots were produced by ‘Shaary’ explants on culture medium supplimented with BAP at 0.5mgl-1 (39.87mm), 2mgl-1 (38.83mm). Adding BAP at 0.5mgl-1 to the culture medium increased number of formed leaves/shoot for ‘Aln-Eureka’ and ‘Shaary’, and BAP at 1mgl-1 increased number of leaves/shoots of ‘Aln-Eureka’ and ‘Banziher’. Shoots of ‘Aln-Eureka’ formed roots higher by 27.21%, 43.84% and 75.72% than ‘Shaary’, ‘Shehriu’ and ‘Banzahir’, respectively. Adding a combination of NAA at 0.5mgl-1 and IBA at 1mgl-1 estimulated higher percentages of lemon shoots to form roots (35.42%) followed by IBA at 1mgl-1 (33.33%)

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