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Accueil du site → Master → Italie → Sanitary status of stone fruits in East Anatolia (Turkey) and characterisation of a Turkish Apple Chlorotic Leaf Spot (ACLSV) isolate

Istituto Agronomico Mediterraneo di Bari (IAMB) 1999

Sanitary status of stone fruits in East Anatolia (Turkey) and characterisation of a Turkish Apple Chlorotic Leaf Spot (ACLSV) isolate

Sipahioglu, H. M.

Titre : Sanitary status of stone fruits in East Anatolia (Turkey) and characterisation of a Turkish Apple Chlorotic Leaf Spot (ACLSV) isolate

Auteur : Sipahioglu, H. M.

Etablissement de soutenance : Istituto Agronomico Mediterraneo di Bari (IAMB)

Grade : Master of Science (MS) 1999

Résumé
Field surveys were carried out in the main stone fruit, especially apricot growing areas of East Anatolia to assess the sanitary status of varietal collection, mother blocs and commercial orchards. A total number of 1019 samples were tested by ELISA belonging to 75 different cultivars. The following viruses were identified : ACLSV, PDV, PNRSV. A Turkish isolate of ACLSV from a native apricot variety, differed form the known isolates in its biological properties by not infecting Chenopodium quinoa L. The virus was purified successfully and a polyclonal antiserum was produced by immunising a rabbit. The production of monoclonal antibodies was also started. Protein preparation of ACLS-EA was separated on 12percent SDS-PAGE, than analysed by Western blotting with polyclonal antiserum produced against isolate. A kit ELISA was prepared for double antibody sandwich immunosorbent assay with a titre of 1:500 determined by dilution series test. Immunoelectron microscopy tests demonstrated the decoration of homologous isolate at the dilution of 1:60.

Mots-clés : APRICOTS ; ELECTRON MICROSCOPY ; ELISA ; MOLECULAR HYBRIDIZATION ; MONOCLONAL ANTIBODIES ; PLANT VIRUSES ; TURKEY ; VIROIDS

Présentation (IAMB Library)

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