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Istituto Agronomico Mediterraneo di Bari (IAMB) 2004

Improvement of Citrus exocortis viroid (CEVd) and Citrus cachexia viroid (CcaVd) detection

Abd-Elbacki, Ashraf Mousa Mahmoud

Titre : Improvement of Citrus exocortis viroid (CEVd) and Citrus cachexia viroid (CcaVd) detection

Auteur : Abd-Elbacki, Ashraf Mousa Mahmoud

Etablissement de soutenance : Istituto Agronomico Mediterraneo di Bari (IAMB)

Grade : Master of Science : Integrated pest management of Mediterranean fruit crops 2004

Résumé
Citrus exocortis viroid (CEVd) and Citrus cachexia viroid (CCaVd) detection was improved by setting up a new biological indexing method and comparing different molecular assays (molecular hybridisation, RT-PCR, sPAGE). Selected CEVd and CCaVd - infected sources, previously tested by sPAGE, were inoculated to Etrog citron cuttings, treated for rooting and kept in plastic bag at 35°C. Plants showed Ieaf epinasty 20 days after inoculation and reacted positively to dot-blot tests. This method proved to be reliable, time and space saving, easy and low cost. Comparing molecular assays in field diagnosis, RT-PCR showed to be the most sensitive method for CEVd and CCaVd detection from January to June, while dot-blot, tissue print and sPAGE were limited by the low viroid concentration in winter months. May can be considered the best month for starting viroid field detection, under Apulia conditions. Moreover, flowers showed to be a promising explant for viroid mass detection, mainly by tissue print.

Mots-clés : CACHEXIA ; CITRUS ; DIAGNOSIS ; EXOCORTIS ; FLOWERS ; INOCULATION ; PCR ; TISSUE PRINT ; VIROIDS

Présentation (IAMB Library)

Page publiée le 14 mars 2018, mise à jour le 4 septembre 2018