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Istituto Agronomico Mediterraneo di Bari (IAMB) 2006

Improvement of protocols for citrus psorosis virus (CPsV) elimination and in-vitro virus conservation

Meziane, Malika

Titre : Improvement of protocols for citrus psorosis virus (CPsV) elimination and in-vitro virus conservation

Auteur : Meziane, Malika

Etablissement de soutenance : Istituto Agronomico Mediterraneo di Bari (IAMB)

Grade : Master of Science : Integrated pest management of Mediterranean fruit crops 2006

Résumé
An improved thermotherapy protocol at 40°C was developed for CPsV elimination. In the method applied in vivo, preconditioning was eliminated and the whole procedure was reduced to 13 weeks instead of 24, using chip budding of pretreated buds. Pretreated budsticks of the same infected sources were successfully grown in vitro at the same temperature. Serological and biological assays showed no infection from 9 weeks (in vivo) and 1 month (in vitro) after heat treatment. In vitro conservation of CPsV sources was obtained using 1 mm shoot-tip grafted in vitro onto T. citrange. The infected rootstock was micro- propagated for shoot proliferation without loosing the infection. STG and SE of some citrus genotypes were not successful. Flowers for somatic embryogenesis from stigma and style culture were successfully stored at 4°C for 2 weeks inducing embryo formation, whereas only lemon cuttings were successfully stored at the same temperature for 2 months.

Mots-clés : CITRUS ; DIAGNOSIS ; IN VITRO CULTURE ; SANITATION ; THERMOTHERAPY

Présentation (IAMB Library)

Page publiée le 12 mars 2018, mise à jour le 11 septembre 2018