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Istituto Agronomico Mediterraneo di Bari (IAMB) 2016

Survey and molecular characterization of Pepino Mosaic virus (PepMV) infecting tomato crops in Morocco

Bibi, Imane

Titre : Survey and molecular characterization of Pepino Mosaic virus (PepMV) infecting tomato crops in Morocco

Auteur : Bibi, Imane

Etablissement de soutenance : Istituto Agronomico Mediterraneo di Bari (IAMB)

Grade : Master of Science : Integrated pest management of Mediterranean fruit 2016

Résumé
Pepino mosaic virus (PepMV) is currently causing great concern in the Moroccan greenhouse tomato industry, where it produces important economic losses. A survey was conducted from October 2015 to April 2016 on PepMV in seven different regions throughout Morocco. In a few plants, severe symptoms which could be associated to the virus were observed. A total of 315 samples of different varieties were collected and tested by DAS-ELISA for the presence of PepMV using a commercial kit. The obtained results showed that PepMV was widely distributed in the country with an infection rate of 21%, especially in the region of Souss Massa where 70% of the tested plants were infected. Representative samples from each region were selected and submitted for additional RT-PCR testing ; 40% of the 83 tested samples were infected. Five isolates were selected for molecular characterization by sequencing the coat protein gene ; all the isolates showed high nucleotide similarity with the Chilean CH2 strain (DQ000985), with a sequence homology ranging from 99.2% to 99.8%. These results should be considered for the development of control strategies for PepMV based on cross-protection using mild strains. Furthermore, the present study allowed the first report of Tomato torrado virus in the country.

Mots-clés : DAS-ELISA ; PEPINO MOSAIC VIRUS ; RT-PCR ; SURVEYS ; TOMATOES

Présentation (IAMB Library)

Page publiée le 6 mars 2018, mise à jour le 7 septembre 2018