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Xinjiang Normal University (2017)

Identification and Biological Characteristics of Seven Algae in Taklimakan Desert of Xinjiang

卡丽比努尔·艾依提

Titre : Identification and Biological Characteristics of Seven Algae in Taklimakan Desert of Xinjiang

Auteur : 卡丽比努尔·艾依提;

Grade : Master’s Theses 2017

Université : Xinjiang Normal University

Résumé
The study isolated 4 strains green algae and 3 strains cyanobacteria from Xinjiang Taklimakan desert to identify genetic position by morphological and molecular biological method,to investigate the growth cycle and effects of temperature,light intensity and pH value on the growth of desert algae.analysis MC-LR and microcystin synthetase mcyE gene of desert algae by method of high performance liquid chromatography and detection gene,to provide a theoretical basis for molecular biology and application of algae foods of Xinjiang Taklimakan desert algae.First,The 3YS16(1)is divided into oval algae by optical microscope and electron microscop,3YS21-1、YS2-3 、KXII(2)into spherical algae ;3YS16 is green,thick,oily,small cells and oval algae ;KXII(2)is yellow,dry,hard to pick,cells are relatively large,spherical algae ;3YS21-1 is green,oily,spherical algae ;YS2-3 is yellow,dry,hard to pick,nearly spherical algae.BYS14-1、YS3-1 and JP4-1is Nostoc.Secondly,Molecular biology methods18 S rRNA and 16 S rRNA gene sequence analysis demonstrated that strains 3YS16(1)and 3YS21-1 were identified Chlamydomonas incerta.strain YS2-3 were identified Chlorosarcinopsis communis.strain KXII(2)were identified Chlorosarcinopsis bastropiensi.Strain BYS14-1 were identified Anabaena.Strain YS3-1 were identified Leptolyngbya sp.low homology of sequencing JP4-1 with known GenBank sequence and similarity rate is 95%,so may be JP4-1 is new genus.Again,draw out seven strains of desert algae growth curve and study the effects of external factors on the desert algae.The results showed that 4 strains of algae of the third day entered the logarithmic growth phase.cyanobacteria of BYS14-1 second days into the logarithmic growth phase ;YS3-1 third days into the logarithmic growth phase ;JP4-1on the ninth day after entered the logarithmic period,seven desert algae did not enter the decline phase.Desert algae 3YS16(1)suitable pH vales is the acidic environment,and the optimum pH was 6.0,temperature is 40℃,light intensity is 2500 Lux ;3YS21-1 suitable pH was 5.0,temperature is 40℃,light intensity is 2500 Lux ;KXII(2)optimum pH was 8.0,temperature is 35℃,light intensity is 4500 Lux ;YS2-3 optimum pH was 8.0,temperature is 30℃,light intensity is 2500 Lux ;BYS14-1 optimum pH was 5.0,temperature is 30℃,light intensity is 3500 Lux ;YS3-1 optimum pH was 5.0,temperature is 30℃,light intensity is 3500 Lux ;JP4-1 optimum pH was6.0,temperature is 40℃,light intensity is4500 Lux.Finally,by high performance liquid chromatography(HPLC)and PCR gene detection method for microcystin MC-LR and microcystin synthetase gene mcyE,HPLC results showed that microcystin standard retention time is 17.8min,but 7 strains desert algae without reservation.The PCR gene detection results showed that primers did not amplified the E gene sequences.The results showed that these 7 strains of desert algae have not microcystin synthetase gene,indicating that the 7 strains of algae did not produce algal toxins.The results were consistent with the results of high performance liquid chromatograpHy for detection of microcystin MC-LR.The results showed that the algae isolated from the Taklimakan Desert did not produce algal toxins

Mots clés : Taklimakan Desert; Desert algae; Molecular identification; Microcystins; external factors;

Présentation (CNKI)

Page publiée le 11 février 2018