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Accueil du site → Doctorat → États-Unis → 1977 → NUTRIENT ANALYSIS OF PRICKLY PEAR (OPUNTIA FICUS INDICA, LINN)

University of Arizona (1977)

NUTRIENT ANALYSIS OF PRICKLY PEAR (OPUNTIA FICUS INDICA, LINN)

Teles, Francisco Franco Feitosa

Titre : NUTRIENT ANALYSIS OF PRICKLY PEAR (OPUNTIA FICUS INDICA, LINN)

Auteur : Teles, Francisco Franco Feitosa

Université de soutenance : University of Arizona

Grade : Doctor of Philosophy (PhD) 1977

Résumé
The prickly pear cactus (Opuntia ficus indica, Linn.) was studied in respect to its use for human and/or animal consumption. The analyses were carried out by chemical and/or physical means. The gross composition was determined by standard procedures, and its nutritional value for humans was comparable with lettuce, egg plant, or other high moisture containing vegetables. Atomic spectrophotometry and colorimetry were used for individual mineral determinations, including calcium, magnesium, phosphorus, potassium and sodium. Pigments and/or vitamins were determined chemically, with most of the work done by column chromatography followed by colorimetry, turbidimetry, and spectrophotometry. Usually more than one method was used for each determination and the results compared statistically. Individual amino acids were analyzed by automated amino acid analyzer with the protein score and biological value being determined mathematically. A new technique was developed for analysis of total nonstructural carbohydrates, and the individual mono and disaccharides were analyzed by gas-liquid chromatography. Analyses of the component fatty acids of the individual lipid fractions : phospholipids, glycolipids and neutral lipids were carried out by column chromatography followed by gas-liquid chromatography. Cellulolytic activity was comparatively determined in different substrates and certain beta-glucanase activity was found in fresh preparations of prickly pear juice. The crassulacean-type acid metabolism was confirmed by gas chromatography of the silanized organic acids on samples collected before and after dark. Historical, botanical and economical aspects of prickly pear cultivation were discussed based on its usefulness as food for human and/or animal consumption.

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