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Accueil du site → Doctorat → Chine → 2009 → Anatomical and Physiological Characteristics of Eco-adaptability of Heteromorphic Leaves in Populus Euphratica Oliv

Beijing Forestry University (2009)

Anatomical and Physiological Characteristics of Eco-adaptability of Heteromorphic Leaves in Populus Euphratica Oliv


Titre : Anatomical and Physiological Characteristics of Eco-adaptability of Heteromorphic Leaves in Populus Euphratica Oliv

Auteur : 岳宁;

Grade : Doctoral Dissertation 2009

Université : Beijing Forestry University

Résumé partiel
Populus euphratica Oliv is the only naturally-distributed tall arbor species in arid desert regions of northwestern China and Central Asia.The tolerance to severe drought and high salinity,high alkalinity soil makes it ecologically important in protecting species diversity and maintaining the ecological balance.The long term adaptation to such desert environment induces the formation of heteromorphic leaves of Populus euphratica,and a single adult tree commonly has ploymophic leaves,such as lanceolate,oval and serrated broad-oval leaves.Special research interest has arisen in understanding the eco-adaptability of Populus euphratica for several years.Comparing the structural and physiological characteristics of the heteromorphic leaves and studying on the relative molecular regulatory mechanism would have both theoretical and practical significance in this area.This paper studied three typical heteromorphic leaves of Populus euphratica,including lanceolate, ovate and serrated broad-oval leaves,and mainly focused on the leaves’ anatomical structures, physiological characteristics and proteomics.It will greatly help the understanding of cellular and molecular mechanism during leaves development and elucidating the mechanism of the eco-adaptability of Populus euphratica.Environmental conditions bave close relationships with leave growth and development.Different leaf shape and anatomical structure characteristics can reflect its adaptive capacity to its habitats. Experiments were conducted in leaf shape morphology and anatomical structure of Populus euphratica. The results showed that as the leaf shape changed from lanceolate to serrated broad-oval,the leaf width increased,surface area decreased,the level of carnification enhanced,and petiole extended ;the serrated broad-oval leaves had the most well-developed subcortex and palisade tissue,as well as the thickest corneum,while lanceolate leaves’ xeromorphic structure was not as well-developed as serrated broad-oval leaves.This demonstrated that the serrated broad-oval leaves were more suitable for arid environments survival compared to the other two types of leaves.The capability of tolerating adverse environment and photosynthesis of leaves can be reflected by several physiological indexes,such as the levels of water potential,bound water,osmolyte,and chlorophyll.Those measurements showed that these three types of leaves had the similar amounts of water,but the serrated broad-oval leaves had lowest water potential,the highest level of bound water, osmolyte and chlorophyll,which explained why the serrated broad-oval leaves had strongest capability of photosynthesis and tolerating adverse environment.The soluble proteins of heteromorphic leaves were separated by SDS-PAGE.Established a suitable Tris-HCl buffer:5%β-mercaptoethanol,2%SDS,0.05mol/L Tris-HCl(pH8.8),10%glycerin, 1mmol/L PMSF。The results indicated that protein expressions were different among leaves in different shapes.

Mots clés : Populus euphratica Oliv; heteromorphic leaf; proteome; 2-dimensional electrophoresis; tandem mass spectrum;

Présentation (CNKI)

Page publiée le 16 mai 2018