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Istituto Agronomico Mediterraneo di Bari (IAMB) 1995

The characterization of five isolates of olive latent virus 1 (OLV-1) and its relationship with tobacco necrosis virus (TNV)

MERCIECA Vanessa

Titre : The characterization of five isolates of olive latent virus 1 (OLV-1) and its relationship with tobacco necrosis virus (TNV)

Auteur : MERCIECA Vanessa

Etablissement de soutenance : Istituto Agronomico Mediterraneo di Bari (IAMB) - Centre international de hautes études agronomiques méditerranéennes (CIHEAM

Grade : Master of Science : Integrated pest management of Mediterranean fruit and vegetable crops 1995

Résumé
Four new isolates of olive latent virus 1 (OLV-1) which was first recorded from Apulia, were recovered from other geographical regions -Tuscany, Sicily, Jordan and Turkey. The Turkish isolate was obtained from Mexican lime, a different host for this virus, other than olive. Preliminary work also indicated that OLV-1 is related with tobacco necrosis virus (TNV). Thus the objectives of this work were : a) to carry out a comparative study of the five viral isolates ; b) to identify the extent of correlation between OLV-1 and the two serotypes of TNV ; c) to identify serological diagnostic techniques which can give rapid and reproducible results. Biological, physicochemical and serological characterization indicate that the five isolates of OLV-1 are identical. Main differences between TNV and OLV-1 were the type of infection caused by TNV on N. benthamiana, a lighter coat protein and the presence of a satellite virus with both TNV-A and TNV-D. A distant serological correlation between OLV-1 and TNV was evidenced in immunodiffusion tests, ELISA and electron microscopy. The results indicate the possibility that OLV-1 is a possible member of the necrovirus group and not a sobemovirus as previously indicated. Polyclonal and monoclonal antibodies were produced, however an attempt to identify a suitable serological technique for reliable diagnosis of OLV-1 in woody plants failed. Further work is necessary to characterize the produced monoclonal antibodies and to try to set up an appropriate serological diagnostic technique for this virus.

Document source : page 235

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