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Accueil du site → Master → Italie → Evaluation of the sanitary status of grapevine in Jordan and biological, serological and physico-chemical comparison of some Gflv isolates

Istituto Agronomico Mediterraneo di Bari (IAMB) 1998

Evaluation of the sanitary status of grapevine in Jordan and biological, serological and physico-chemical comparison of some Gflv isolates

AL-TAMINI Naser

Titre : Evaluation of the sanitary status of grapevine in Jordan and biological, serological and physico-chemical comparison of some Gflv isolates

Auteur : AL-TAMINI Naser

Etablissement de soutenance : Istituto Agronomico Mediterraneo di Bari (IAMB) - Centre international de hautes études agronomiques méditerranéennes (CIHEAM

Grade : Master of Science : Integrated pest management of Mediterranean fruit and vegetable crops 1998

Résumé
Surveys were carried out in commercial vineyards and in mother plots in the main grapevine growing areas of Jordan (High land, Jordan Valley, Desert areas) to assess the presence and the incidence of virus diseases. Field inspections for the collection of samples were conducted in winter 1997. Biological (sap inoculation herbaceous plants and graft-transmission on woody indicators) and serological (different ELISA procedures) tests were performed to this aim. About 60% of the ELISA tested vines were infected by one or more viruses (GFLV, GVA, GVB, GFkV, GLRaV-1, -3, and -7). Vein necrosis symptoms were also observed on graft-inoculated 110R plants, whereas no mechanically transmissible viruses other than GFLV were recovered in inoculated herbaceous hosts. In a comparative study of some GFLV isolates, no serological differences were observed in double diffusion tests in agar gel. Similarly, there was an apparent uniformity in the molecular weights of their RNAs, except for one Turkish isolate that showed the presence of a satellite. Slight differences in the CP molecular weights were shown by two Italian isolates in comparative migration rate in SDS-PAGE.

Document source : page 243

Page publiée le 25 septembre 2018