Informations et ressources scientifiques
sur le développement des zones arides et semi-arides

Accueil du site → Doctorat → Allemagne → 2018 → Genetic dissection of shoot traits and proline content under control and drought conditions in barley

Rheinischen Friedrich-Wilhelms-Universität Bonn (2018)

Genetic dissection of shoot traits and proline content under control and drought conditions in barley

Shumaila Muzammil

Titre : Genetic dissection of shoot traits and proline content under control and drought conditions in barley

Auteur : Shumaila Muzammil

Université de soutenance : Rheinischen Friedrich-Wilhelms-Universität Bonn

Grade : Doktorin der Agrarwissenschaften (Dr. agr.) 2018

Résumé
Drought is the most severe threat to world crop cultivation and production especially in water shortage areas. Wild barley diversity contains notable variation in phenotype that is essential for its adaptation to abiotic stress like drought. In the current study, we performed QTL mapping for shoot traits and proline content accumulation under control and drought conditions. A library of 73 (BC3S4:S10) S42ILs derived from German cultivar Scarlett and wild accession from Israel (ISR42-8) was used in this experimental study and genotyped for shoot traits with a 1,536-SNP Illumina BOPA1 set. Plants were analyzed and phenotypic data was collected for eight shoot traits and physiological trait i.e ; proline content. All studied traits showed high significant differences between both treatments. Genetic mapping reveals total twenty QTLs for shoot traits and five QTLs for drought inducible proline accumulation all over the barley genome and had main effects on improving or reducing the traits under control and drought stress conditions. The most important QTL which have been obtained in the current study is for proline content on 1H chromosome. Further mapping and validation in a high resolution population revealed that Qpro.S42-1H underlie a previously unknown HvP5CS1 allele originated from wild barley. The functional mutations were found in the promoter motifs for DNA binding transcription factor i.e ; ABRE-binding factors (ABF1, ABF2), where the number and arrangements of ABFs binding motifs in the wild P5CS1 allele in ISR42-8 appeared to imply transcriptional up regulation and excessive proline accumulation under extreme drought conditions. Higher proline accumulation in QTL allele bearing ILs S42IL-143 and S42IL-141 conferred improved physiological activity and photosynthetic yield, thus confirming functionality of an exotic P5CS1 allele in the cultivated barley. The present findings brought up a first insight on the molecular and evolutionary regulation of an essential drought physiological traits in crop plant. These resources offer opportunity to understand adaptive biology of crop plants and can serve as direct target for trait improvement in barley and related species.

Présentation

Version intégrale (5 Mb)

Page publiée le 23 novembre 2018, mise à jour le 31 décembre 2018