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Accueil du site → Master → Soudan → In vitro Assessment of Acaricidal Activity of Hot water Extract of Citrullus colocynthis (Alhandal) Fruit, Azadirchta indica (Neem) leaves and Allium sativum (Garlic) Cloves against Hyalomma anatolicum larvae

University of Khartoum (2017)

In vitro Assessment of Acaricidal Activity of Hot water Extract of Citrullus colocynthis (Alhandal) Fruit, Azadirchta indica (Neem) leaves and Allium sativum (Garlic) Cloves against Hyalomma anatolicum larvae

Mohamed, Asma Gibril Eljack

Titre : In vitro Assessment of Acaricidal Activity of Hot water Extract of Citrullus colocynthis (Alhandal) Fruit, Azadirchta indica (Neem) leaves and Allium sativum (Garlic) Cloves against Hyalomma anatolicum larvae

Auteur : Mohamed, Asma Gibril Eljack

Université de soutenance : University of Khartoum

Grade : Master of Science (MS) 2017

Résumé
This study was conducted to assess the acaricidal activity of the hot water extract of Citrullus colocynthis fruit, Azadirachta indica leaves and Allium sativium cloves against Hyalomma anatolicum larvae. The three plants were collected from khartoum and were authenticated by botanist in the Medicinal and Aromatic Plants Research Institute. (MAPRI), Khartoum. The materials of plants were dried in shaded ventilated area at room temperature for one week before grinding to powder for extracted. Phytochemical analysis for active ingredients from hot water extract of three plants was carried out on the Department of Chemistry in (MAPRI). Engorged female ticks were collected from naturally infested cattle in Elgreif west dairy farms and were identified as H.anatolicum in the Department of Entomology and Ticks, Central veterinary Research Laboratory (CVRL) .Ticks were kept in desiccators over saturated sodium chloride solution to provide 75-80% relative humidity (RH) .The desiccator was maintained in an incubator at 27±1οC to provide optimum conditions for oviposition. Different concentrations (40%, 20%, 10% and 5%) of plants extracts were prepared and the control solution was set up with distilled water. Three to Five hundred ,12-14 days old unfed larvae of H.anatolicum were used to evaluate the acaricidal activity of the candidate plants extract using Larval Immersion Test (LIT), for 48hr after which alive and dead larvae were counted and calculate the larvae mortality rate corresponding to each concentration used. If there was mortality in the control group more than 5% larval percentage rates achieved were corrected. Data were expressed as mean ± SEM and were compared using X one-way ANOVA. The results of phytochemical analysis of the three plants showed the presence of flavonoids, alkaloids, cumarins, tritrepens saponins, and anthraquinone glycosides, tannins (absent in C. colocynthis) and steroids (absent in Allium sativum). The hot water extracts of Citrullus colocynthis fruit , Allium sativum cloves, and Azadirchta indica leaves showed toxicity against H.anatolicum larvae , there was significant (p<0.05) high mortality rate of larvae in high concentration (40%) was 80.9%, 67.8% and 16.8% respectively. It was concluded that the hot water extracts Citrullus colocynthis fruit Allium sativum cloves, and Azadirchta indica leaves of proved to have larvicidal efficacy with the mortality rates increased with increasing concentration of extracts, with C.colocynthis having the best acaricidal activity among others. I was recommended conduct further studies during other life cycle stages of Hyalomma anatolicum and use other types of plant extracts. The study also recommended develops these plants to be in use as acaricidal agents replacing chemical drugs to minimize their adverse effects on host and environment.

Présentation

Page publiée le 22 novembre 2018