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University of Gezira (2011)

In vitro Study of Antifungal and Antiaflatoxin Activity of Lemongrass (Cymbopogon nervatus (Hochst.) chiov.) Essential Oil Against Aspergillus flavus and Aspergillus niger

Merghani, Iyman Yousif Ahmed

Titre : In vitro Study of Antifungal and Antiaflatoxin Activity of Lemongrass (Cymbopogon nervatus (Hochst.) chiov.) Essential Oil Against Aspergillus flavus and Aspergillus niger

Auteur : Merghani, Iyman Yousif Ahmed

Université de soutenance : University of Gezira

Grade : Doctor of Philosophy, in Biosciences and Biotechnology 2011

Résumé
Cymbopogon nervatus, of the family Graminae, is an aromatic plant that grows wild in Sudan especially at Butana region, this plant has many claimed folk medicinal benefits and is locally used for treatment of indigestion and kidney troubles. Also most of the Sudanese in rural areas use it as building material for roofing and fencing.. In the present study the essential oil of C. nervatus was extracted by using the steam distillation and the antifungal activity of the extract was evaluted against A. flavus and A. niger invitro, also studied was the effect of the essential oil on Aflatoxin production by A.flavus. The different chemical constitunets of the essential oil were seperated using thin layer chromatography (TLC), eluted from the plates and each individually tested for antifungal activity against A. flavus. The bioassay was carried out in vitro using the disk diffusion method. Further separation and identification of the essential oil components, including anti-fungally active ones, was carriedout using GC/MS. Essential oil concentrations of 50% and 40% (equal to 10 and 8 µl) resulted in complete inhibition of the growth of both test organisms and reduction of Aflatoxin production by A. flavus. Moreover, a dose of 20 µl/ml was completely protected storage grains against A. flavus more than 28 days. Two of the eight separated components of the essential oil of C. nervatus showed antifungal activity against A. flavus. Mass spectra and GC/M data analysis pointed to the active compounds had alcoholic moiety. The major compound of C. nervatus essential oil is estragole (34.5%). Further studies need to be carried out so that the essential oil of C. nervatus could be used on a commercial scale as a fungicide for protecting storage grains

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