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University of Gezira (2013)

Chemical Composition of Lantana (Lantana camara L.) Leaves Essential Oil and its Antimicrobial Activity

Idris, Nosiba Hassan Elsheikh

Titre : Chemical Composition of Lantana (Lantana camara L.) Leaves Essential Oil and its Antimicrobial Activity

Auteur : Idris, Nosiba Hassan Elsheikh

Université de soutenance : University of Gezira

Grade : Doctor of Philosophy in Oil Chemistry 2013

Résumé
Lantana camara (L), of the family Verbenaceae, is listed as one of the important medicinal plants of the world. The plant is an evergreen aromatic plant, found in tropical and subtropical areas around the world. In Sudan it is commonly grown as an ornamental plant and displays several flower colors combing different shades of red- yellow- purple- violet- white- orange and yellow and orange. The essential oil of Lantana camara showed a wide spectrum of antibacterial, antimicrobial, and antifungal activities. The aims of this study were to evaluate chemical composition of locally grown Lantana plants, to evaluate their content of leaf essential oil and to assay biological activities of crude and purified constituents of the essential oil against a selected bacterium and a fungus. The plants, collected from the Gezira area around Wad-Medani, were extracted by using hydro- distillation method and cold extraction technique. The crude essential oil extracts of lantana Camara Var. aculeata were tested for growth inhibition of Agrobactrium tumefaciens and Aspergillus niger using the disc diffusion method and well fungal- growth disc diffusion method. Chemical fractionations and component identification were performed using TLC, PTLC, GC/MS, MS, UV, and FTIR. The results indicated that the crude essential oils of lantana Camara Var. aculeate possessed considerable antibacterial activities, with lethal concentration (LC50) between 160-200μl/ml and minimum inhibitory concentration (MIC) between 4 and 8 μl/ml. On the other hand, the essential oil showed significant antifungal activity resulting in 100% kill at doses of 280 and 300 μl/ml, and LC50 of 160μL/ml, and 8-12μL/ml for MIC. Of nine separated components, eluted from PTLC-plate, only two (C1 and C6) were found active against A. niger (causing antifungal inhibition of 14% and 55% respectively). The major constituents of the essential oil of Lantana camara dependent on GC/MS were limonene, C6-Ketone, the sesquiterpene -cayrophyllene, representing more than 30% of the oil. Compared with Iran(14%), China(12%), and Nigeria(13%).

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