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University of Gezira (2015)

Phytochemistry and larvicidal potentiality of the local usher (Calotropis procera Ait) flower part against mosquitoes (Diptera : Culicidae) larvae

Abbas, Alia Ibrahim Bashir

Titre : Phytochemistry and larvicidal potentiality of the local usher (Calotropis procera Ait) flower part against mosquitoes (Diptera : Culicidae) larvae

Auteur : Abbas, Alia Ibrahim Bashir

Université de soutenance : University of Gezira

Grade : Master of Science (MS) In Biosciences and Biotechnology 2015

Résumé
Phytochemistry and larvicidal potentiality of the local usher (Calotropis procera Ait) flower part against mosquitoes (Diptera : Culicidae) larvae Alia Ibrahim Bashir Abbas Abstract Usher plant is one of the many plants known for its pharmaceutical importance and with its toxicity against mosquitoes larvae and some other insects. It is also of those widely distributed plants in the area that is not much used. This study aim to run a comparative study on some qualitative phytochemical analysis for the main classes, thin layer chromatography (TLC) and biological test produced by the extracts) characteristics of usher flower parts (petals, corona, gynostigium, style and ovary). The usher flower samples were collected from Nishishiba area (the main campus, University of Gezira), whereas, Culex and Anopheles larvae were collected from the breeding sites of Tayba area and Karkog villige in March 2014. All chemical and biological tests were run at the Basic Sciences Laboratory and Food Analysis Laboratory, University of Gezira. The result of this revealed that, each of the polar and apolar extracts separated only two spots for each usher flower parts. The tannins, flavonioids, glycosides and steroids were detected in the petals and corona flower parts, while gynostigium and the ovary and style parts possessed only flavonoid and sterols. The mean mortality produced by the petals, corona, gynostigum and ovary and style flower parts were 87.5 % , 100% , 95% and 20$ respectively. A mean of 71.25% of Anopheles larvae were killed by all usher flower parts, while 80% of the Culex larvae were killed, but A nova did not prove a significant difference between the susceptibility of both larvae. It was recommended that, corona, gynostigium and petals of usher flower parts can used as potential sources to control mosquito larvae, since that they were potential, available, cheap, simply to be prepared.

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