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University of Gezira (2015)

Qualitative Phytochemical Screening, Thin Layer Chromatography and Toxicity Tests for the Stems of Two Cactus Genera : Euphorbia trigona and E. abyssinica

Hamid, Hanadi Alwan Ali Abd Alla

Titre : Qualitative Phytochemical Screening, Thin Layer Chromatography and Toxicity Tests for the Stems of Two Cactus Genera : Euphorbia trigona and E. abyssinica

Auteur : Hamid, Hanadi Alwan Ali Abd Alla

Université de soutenance : University of Gezira

Grade : Master of Science (MS) in Biosciences and Biotechnology 2015

Résumé
There are over 2000 species of Euphorbia (Euphorbiaceae) in the world. The plants are succulent and the aerial parts of some species exude a milky fluid that exerts a number of physiological and a wide range of biological properties, including antioxidant, anti-inflammatory, pesticidal, cytotoxic, antibacterial and multi-drug resistance reversing activities. The objective of this study was to run qualitative phytochemical screening, thin layer chromatography (TLC) and toxicity tests for the stems of two cactus genus : Euphorbia trigona and E. abyssinica. Stems of E. trigona, were collected from within Wad Medani Town, Gezira State, whereas one large stem of E. abyssinica was brought from Erkwit mountain, Red Sea State. All of the laboratory tests were done in the Food Analysis Laboratory, Faculty of Engineering and Technology, University of Gezira. The collected stems of both plants were firstly dissected into outer parts (cortex) and inner part (pith). These parts were dried, grounded and then subjected to phytochemical screening (presence of the main classes), TLC and toxicity tests, using recommended methods. The results of this study showed that, all plant samples contained the same phytochemicals similarly, but with different concentrations for some classes, even in the same plant. The TLC tests of each of E. trigona and E. abyssinica, separated one spot from each of the stem pith and cortex per each extract (polar and apolar), but with different Rf values. ANOVA proved that, the susceptibility of Anopheles and Culex larvae toward Euphorbia stem parts (at 1.2 mg/L) were not similar, and it also proved that, the toxicity of the used plant parts were statistically not similar. The recommendations of this study were to run proper chemical and phytochemical tests (instead of some tests). An antimicrobial and pharmaceutical activity tests should be run, and also to run a proper toxicity test.

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