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Universidad Nacional de Trujillo (2016)

Determinación de bacterias presentes en la rizósfera de Opuntia ficus-indica var. inermis en suelos áridos de Tumbes, Perú.

Mendez Farroñan, Sandra Johana

Titre : Determinación de bacterias presentes en la rizósfera de Opuntia ficus-indica var. inermis en suelos áridos de Tumbes, Perú.

Auteur : Mendez Farroñan, Sandra Johana

Université de soutenance : Universidad Nacional de Trujillo

Grade : Título Profesional Biologo 2016

Résumé
Research on the microbiota exists in the rhizosphere of plants and their beneficial effects on the development and promotion of growth thereof, have now been favorite subjects for researchers, so that the objective of this research is aimed at determining bacteria present in the rhizosphere of Opuntia ficus-indica in arid soils of Tumbes, Peru. The biological material came from the rhizosphere of Opuntia ficus - indica. The cultivation of isolates processed in Trypticase Soy Broth and Luria Bertani, to which was made DNA extraction, then the PCR technique was applied ; whereby the amplicons of the treated samples were obtained ; Finally respective electrophoresis and sequencing the 16S rDNA was performed, and the method of metagenomics cultivable and non-cultivable bacteria was identified in the rhizosphere ; The data analysis was done using the program MG- RAST were consulted BLAST of NCBI to identify the species, it is concluded that was possible to identify 46 species of cultivable bacteria in the rhizosphere of Opuntia ficus - indica, using metagenomics identification of arable and non-arable these native bacteria in the rhizosphere, being about 600 genera per sampling point was achieved. Keywords : rhizosphere, Opuntia ficus-indica, metagenomics.

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