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Universidad Nacional de Trujillo (2016)

Hongos Micorrizicos Arbusculares nativos presentes en la rizósfera de Opuntia ficus-indica en suelos áridos de Tumbes, Perú.

Quezada Salirrosas, Marilyn Rosa Alexandra

Titre : Hongos Micorrizicos Arbusculares nativos presentes en la rizósfera de Opuntia ficus-indica en suelos áridos de Tumbes, Perú.

Auteur : Quezada Salirrosas, Marilyn Rosa Alexandra

Université de soutenance : Universidad Nacional de Trujillo

Grade : Título Profesional Biologo 2016

Résumé
40% of the surface of Peru corresponds to arid areas. Currently some strategies are oriented to turn them into arable land. typical of these ecosystems species, such as O. ficus-indica cactus, they are very efficient in water use. The association with mycorrhizal fungi Arbusculares (HMA) from its rhizosphere gives resistance to adverse factors how are you arid areas. This experience aimed to isolate and identify native HMA present in the rhizosphere of O. ficus-indica in arid soils of Tumbes, Peru. rhizosphere samples were obtained at 5 arid Tumbes, where spores of HMA was isolated by sieving method and then multiplied in alfalfa crop traps. The molecular analysis of HMA began with the extraction of DNA, then proceeded with testing semi-nested PCR using external primers (NS5 + ITS4) and specific (NS5 + GLOM5.8R ; ITS4 + GLOM1310 ; NS5 + GIGA5.8R ), these tests were evaluated by electrophoresis. The results showed positive bands in the samples problems, this suggests that using semi-nested PCR is possible to identify HMA in the rhizosphere associated with O. ficus-indica.

Mots clés Arbuscular mycorrhizal fungi, rhizosphere, O.ficus-indica, arid soil.

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Page publiée le 18 janvier 2019