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King Abdulaziz University (2017)

Morphological, Biochemical and Molecular Evaluation for four genotypes of the genus Pandanus

Almutire, Soliman Turki

Titre : Morphological, Biochemical and Molecular Evaluation for four genotypes of the genus Pandanus

التقييم المورفولوجي، الكيموحيوي والجزيئي لأربعة تراكيب وراثية لجنس البندانس

Auteur : Almutire, Soliman Turki

Université de soutenance : King Abdulaziz University

Grade : Doctorate Thesis 1438 AH 2017 AD

Résumé
The present study considered the morphological, anatomical and molecular characterizations of four Pandanus species, Pandanus tectorius var ‘Utilis’, Pandanus tectorius var ’Variegata’ and Pandanus dubius were obtained from the local market in Jeddah, Saudi Arabia), and Pandanus odoratissimus naturally grown from three different regions of Saudi Arabia. The experiments were conducted during 2015-2016 in the Lab of Plant Tissue Culture and the Lab of Plant Biotechnology, Department of Arid Land Agriculture, Faculty of Meteorology, Environment and Arid Land Agriculture, King Abdulaziz University. Morphological and anatomical characterizations were conducted using three leaves types, old, medium and new leaves of 15 pandanus seedlings of each species. Molecular chrachtarization was conducted using DNA samples isolated from new leaves of 5 seedlings of each species. RAPD-Based PCR technique and 60 random oligonucleotide primers were used to differentiate between Pandanus species. The experiments were laid out in Completely Randomized Design (CRD) using 4 replicates. The results of morphological characterizations revealed that Pandanus tectoruis var Utilis produced leaves with the highest length and ley number of medium age (leaves found in the meddile layers of plant crown) registered the highest length. Highest leaves breadth was observed for Pandanus tectoruis var Variegata and medium age leaves and new young leaves were the widest leaves. Medium leaves of Pandanus tectoruis var Variegata revealed highest thickness followed by old leaves of Pandanus tectoruis var Variegata. Highest leaves fresh and dry weights were found for medium age leaves. Leaves of the local Pandanus species (Pandanus Odoratissimus) attained highest Ch a contents, and medium age leaves attained highest Ch a contents as compared to old and new young leaves. For Ch b highest contents were found in leaves of medium age of Pandanus Odoratissimus, followed by medium leaves of Pandanus tectoruis var Variegata. The new young leaves revealed highest contents of N(%) and (K%), and leaves of Pandanus tectoruis var Variegata contained highest percentage of P. Regarding anatomical characterization of Pandanus species, the results revealed that medium leaves of Pandanus tectoruis var Variegata and medium and old leaves of Pandanus tectoruis var Utilis comprised the highest thickness of epidermis. Highest number of stomata found on surface of new young leaves of Pandanus Odoratissimus, while old leaves of Pandanus tectoruis var Variegata comprised least number of stomata. Old leaves of Pandanus Odoratissimus comprised highest length of spinney and medium leaves of Pandanus tectoruis var Utilis contained least spinney length. Maximum thickness of spinney found on old leaves of Pandanus dubius. Regarding molecular identification of Pandanus species using RAPD-Based PCR markers, the results revealed that a total of 19 polymorphic patterns were successful differentiated the four tested panadanus species. The RAPD specific locus OPC-01 identified the genomes of Pandanus odoratissimus, Pandanus dubius and Pandanus tectorius var ‘Utilis at a site of 480 bp. OPK-10-250 was considered as marker specific to Pandanus dubius, Pandanus tectorius var ’Variegata’ and Pandanus tectorius var ‘Utilis genomes. OPT-04-120 specific for Pandanus dubius, OPF-02-320 for Pandanus odoratissimus, OPC-01-180 was tightly linked to DNA samples of Pandanus odoratissimus and Pandanus tectorius var ‘Utilis, OPF-02-600 was specific for Pandanus odoratissimus and Pandanus dubius, and OPE-08-430 was specific for Pandanus dubius and Pandanus tectorius var ‘Utilis. High similarities were found between Pandanus dubius and Pandanus tectorius var ‘Variegata’ (0.346) and Pandanus tectorius var ’Variegata’ and Pandanus tectorius var ‘Utilis (0.346).

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Page publiée le 20 janvier 2019