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Accueil du site → Doctorat → Turquie → INVESTIGATION OF THE CHROMIUM(VI) TOLERANCE AND THE ROLES OF CHROMIUM(VI) AND SULFATE ON THE PROTEOME CHANGES IN CANOLA (Brassica napus L.) SEEDLINGS

AFYON KOCATEPE UNIVERSITY (2014)

INVESTIGATION OF THE CHROMIUM(VI) TOLERANCE AND THE ROLES OF CHROMIUM(VI) AND SULFATE ON THE PROTEOME CHANGES IN CANOLA (Brassica napus L.) SEEDLINGS

Terzi, Hakan

Titre : INVESTIGATION OF THE CHROMIUM(VI) TOLERANCE AND THE ROLES OF CHROMIUM(VI) AND SULFATE ON THE PROTEOME CHANGES IN CANOLA (Brassica napus L.) SEEDLINGS

Kanola (Brassica Napus L.) Fidelerinde Krom(Vı) Toleransı ve Proteom Değişimleri Üzerine Krom(Vı) ve Sülfatın Rolünün Araştırılması

Auteur : Terzi, Hakan

Université de soutenance : AFYON KOCATEPE UNIVERSITY

Grade : DOKTORA TEZİ 2014

Résumé partiel
In the first stage of this study, the phytoremediation potential of 8 canola (Brassica napus L.) cultivars (Champlain, Dante, Heros, Nelson, NK Petrol, Sary, Süzer and Vectra) for hexavalent chromium [Cr(VI)] were evaluated in hydroponic culture. For this purpose, the alterations in seedling growth, Cr accumulation, malondialdehyde (MDA) content, chlorophyll content, non-protein thiol content (NPT) and activities of some antioxidant enzymes [superoxide dismutase (SOD), guaiacol peroxidase (POD) and catalase (CAT)] were investigated in canola seedlings exposed to Cr(VI) stress (0, 10, 50 and 100 µM potassium dichromate) for 7 days. Chromium stress caused a reduction in dry weights (DW), chlorophyll content and CAT activity, and increase in lipid peroxidation, NPT content and activities of SOD and POD. Based on the dry weight, the tolerance index was found maximum in cultivar NK Petrol (%82.5-74.1) and minimum in cultivar Sary (%65.7-40.8). The cultivar Sary accumulated the maximum amount of Cr (705.8 μg g1 DW), which was correlated with the lowest levels of chlorophyll content and highest levels of lipid peroxidation. However, Cr accumulation was found lowest (255.0 μg g1 DW) in NK Petrol. Moreover, the cultivar NK Petrol maintained high content of chlorohyll and CAT activity under Cr(VI) stress. Although NK Petrol may be a Cr(VI) excluder cultivar relative to Sary, it may have the potential for the phytoremediation of Cr-contaminated sites as it tolerates Cr(VI) stress by producing higher biomasses. In the second stage of this study, the interactions between sulfur (S) nutrition and Cr(VI) stress were evaluated in relatively Cr-tolerant (NK Petrol) and Cr-sensitive (Sary) canola cultivars differing in Cr(VI) tolerance. The canola seedlings were grown S-sufficient (1 mM S) nutrient solution for 7 days. The seedlings were then grown in variable S concentrations [0 mM S (–S), 1 mM S (+S) and 2 mM S (+HS)] for another 7 days. The fourteen-day-old seddlings were exposed to 6 different treatments for 3 days : (1) +S/−Cr ; 1 mM sulfate, 0 μM Cr(VI) (control), (2) +S/+Cr ; 1 mM sulfate, 100 μM Cr(VI), (3) −S/−Cr ; 0 mM sulfate, 0 μM Cr(VI) (sulfur deficiency), (4) −S/+Cr ; 0 mM sulfate, 100 μM Cr(VI) (combined stress), (5) +HS/−Cr ; 2 mM sulfate, 0 μM Cr(VI) (excess S treatment) and (6) +HS/+Cr ; 2 mM sulfate, 100 μM Cr(VI). After this period, the seedlings were used to evaluate the alterations in growth, Cr accumulation, lipid peroxidation, chlorophyll content, thiol content, cysteine content, ascorbic acid (AsA) and glutathione (GSH) contents, activities of some antioxidant enzymes [SOD, POD, CAT, ascorbate peroxidase (APX), glutathione S-transferase (GST) and glutathione reductase (GR)], expression level of metallothionein protein 1 gene (BnMP1) and leaf proteome.

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Page publiée le 23 janvier 2019