Informations et ressources scientifiques
sur le développement des zones arides et semi-arides

Accueil du site → Master → Turquie → THE INVESTIGATION OF ANTIOXIDANT, ANTIMICROBIAL AND

Bartın Üniversitesi (2018)

THE INVESTIGATION OF ANTIOXIDANT, ANTIMICROBIAL AND

Top, Rabia

Titre : THE INVESTIGATION OF ANTIOXIDANT, ANTIMICROBIAL AND ANTICANCER EFFECTS OF SOME IMPORTANCE MEDICAL PLANTS

BAZI ÖNEMLİ TIBBİ BİTKİLERİN ANTİOKSİDAN, ANTİMİKROBİYAL VE ANTİKANSER ETKİLERİNİN ARAŞTIRILMASI

Auteur : Top, Rabia

Université de soutenance : Bartın Üniversitesi

Grade : Master Thesis 2018

Résumé
For thousands of years, plants have been used for the treatment of many diseases. In addition, in modern pharmacy many plant compounds have an important place in the production of pharmaceutical raw materials or new medicines. In this study, the effects of antioxidant, antimicrobial and anticancer effects in addition to the total phenolic content of fruits collected from plants with Momordica charantia, pepino (Solanum muricatum) and gold strawberry (Physalis peruviana) with medical prescription were investigated in vitro and the biological activities of these species were aimed. In this context, firstly all three plant samples were homogenized in 1:10 (g/ml) in 80% ethanol and then the solvent was evaporated and the remaining extract was prepared in 10 ml of phosphate buffer and the stock kept as an example. The total polyphenol content of the samples was measured spectrophotometrically using Folin-Ciocaltute reactivity. The DPPH free radical scavenging effect of plant samples was determined and antioxidant capacities were determined. The antimicrobial effect of the samples was tested by disc diffusion method using both gram positive and gram negative bacterial strains. In addition, the cytotoxic effects of the samples on human over and breast cancer cell lines (A-2780 and MCF-7, respectively) were determined using MTT method. Results were expressed as mean ± standard deviation and p <0.05 was considered statistically significant. As a result of the study, it was determined that the highest total polyphenol content was in the case of potency and the lowest content was in the case of pepino. All three plant samples revealed the effect of free radical elimination of DPPH dependent predation. Except for the Pepino extract, each of the samples showed low antimicrobial activity on the test microorganisms. Finally, it was determined that all three plant samples showed high cytotoxic effect on both human over and breast cancer cell lines. These results show us that in all three plant samples it has a significantly higher anticancer effect than antioxidant and antimicrobial effects. With the extensive studies to be carried out after that, the mechanism of the effect can be enlightened and contribute to new treatment approaches.

Mots clés : Anticancer activity ; antimicrobial activity ; antioxidan activity ; Momordica charantia ; Physalis peruviana ; Solanum muricatum

Présentation

Version intégrale (1,0 Mb)

Page publiée le 24 janvier 2019