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Benha University (2006)

Effect of biofertilizers application on the productivity of nigella sativa cultivated in desert sandy soils and efficiency of produced seeds against some pathogenic microorganisms

Elsayed, Mahmoud Aly Mohamed

Titre : Effect of biofertilizers application on the productivity of nigella sativa cultivated in desert sandy soils and efficiency of produced seeds against some pathogenic microorganisms

Auteur : Elsayed, Mahmoud Aly Mohamed

Etablissement de soutenance : Benha University .

Grade : Doctor of Philosophy (PhD) in Agricultural Science 2006

Résumé partiel
Application of biofertilizers in order to improve the growth and production of Black cumin (Nigella sativa) was conducted in this work. Two field experiments were carried out in El-Sheikh Zowayed North Sinai governorate, Egypt during the two successive seasons 200112002 and 200212003 to study the effect of using biofertilizers on the productivity of black cumin (Nigella sativa) and investigating the obtained seeds against some pathogens. The soil was amended with compost at the rate of 15 m 3/ feddan in the field experiment. It was supplemented with the recommended doses of Supper phosphate (P205 15.5%) l50Kg/Feddan and potassium sulfate 48% (l00 kg/feddan).The experiment included 4 types of treatments namely uninoculated and inoculated treatments with Azotobacter chroococcum and Bacillus megaterium alone or mixed. Each set of treatments contained three levels of mineral nitrogen fertilization ; zero, 25 and 50 Kg /feddan under drip or rain irrigation. The used bacterial strains were prepared in microbiology lab of Desert Research Center. The soil and plant sampling to determine microbiological and chemical analyses were obtained at three stages of growth namely 60,150 and 210 days after planting. The efficiency of antimicrobial activities of oil content of each treatment against some pathogenic microorganisms was determined. SUMMARY The obtained results can be summarized as follows 1- Generally total microbial counts, densities of Azotobacter and densities of Bacillus megaterium under drip or rain irrigation increased at 60, 150 days and then decreased at 210 days. Also the increasing of the rates of mineral nitrogenous fertilization at 25and 50 Kg N/fed. with inoculation by azotobacters caused increment in the total number of microbial count, Azotobacter and Bacillus megaterium counts as compared with uninoculated treatments at 60 and 150 days. 2- Inoculation with Azotobacter chroococcum alone or mixed with Bacillus megaterium under drip irrigation in the presence of 25 Kg N/feddan gave the highest dehydrogenase activities. The rate of metabolic activity in soils under investigation show that all soil conditioning treatments achieved higher dehydrogenase activities than untreated samples in both seasons. 3- Organic carbon in the soil that was planted with Nigella sativa as affected by biofertilizer continuously decreased in the organic carbon content in all treatments as well as untreated soil up to the end of the experiment. 4- The increasing of mineral nitrogenous fertilization rates with inoculation by Azotobacter chroococcum alone or mixed with Bacillus megaterium under drip or rain irrigation resulted in a significant gradual increase in soil nitrogen content after 60 days from plant age as compared with the same mineral nitrogenous fertilization rates in case of absence of inoculation with Azotobacter chroococcum alone or mixed with Bacillus megaterium. 5-Inoculation of the soil with Azotobacter chroococcum and or Bacillus megaterium under drip or rain irrigation resulted in significant increases of available phosphorus or potassium after 60 days from planting as compared with uninoculated treatments. 6- Increasing of plant age caused an increase of plant heights in general. Increasing of mineral nitrogenous fertilization rates accompanied by dual inoculation with Azotobacter chroococcum and Bacillus megaterium under drip irrigation had a significant effect on increasing plant heights in comparison with uninoculated treatments. 7- The number of branches was increased with using half or full dose of mineral nitrogenous fertilization in the presence of dual inoculation under drip irrigation than individual inoculation or uninoculated treatments. There was not any increment in the number of branches with proceeding age of plant from 150 days. 8- By increasing age of plant, an increment in the fresh and dry weights of the plants was occurred till 210 days. Dual inoculation with Azotobacter chroococcum and Bacillus megaterium under drip irrigation resulted in a great increment in the fresh and dry weights than the uninoculated treat ….

Présentation étendue (EULC)

Page publiée le 2 février 2019