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Benha University (2009)

Molecular genetic studies on some natural medical plants

Injy Mohamed Masoud Soliman

Titre : Molecular genetic studies on some natural medical plants

Auteur : Injy Mohamed Masoud Soliman

Etablissement de soutenance : Benha University .

Grade : Doctor of Philosophy (PhD) in Agricultural Science 2009

Résumé partiel
The present study has been carried out in the Genetics and Cytology Unit, Desert Research Center and Genetics Lab, Faculty of Agriculture, Ain Shams University. The present work aimed to study the following : 1-Detection of biochemical and molecular genetic markers for two medicinal plants (Hyoscyamus muticus L. and Calotropis procera (Ait.) Ait f.) using biochemical and molecular genetic fingerprints.2-Studying genetic variation of these medicinal plants at the biochemical and molecular genetic level. The biochemical markers (protein and isozymes) and the molecular markers (RAPD and ISSR) were used to differentiate and study the relationship and genetic variations among two medicinal plants ; henbane and Ushaar. Henbane’ (Hyoscyamus muticus L.) was collected from three different locations (Wadi Sudr, El-Maghara and Shalateen). Ushaar (Calotropis procera (Ait.) Ait. f.) also was collected from three different locations (El-Maghara, Shalateen and Sant Kathrin).1. The obtained results from protein electrophoresis (SDS-PAGE) in henbane (Hyoscyamus muticus L.) : Three henbane ecotypes were fingerprinted by SDS-PAGE of protein. They exhibited a maximum number of 13 bands which were not necessarily present in the three ecotypes in a range of 68.05 to 230.40 KDa. Results revealed that henbane ecotype from Wadi Sudr has two positive bands at the molecular weights ; 202.03 and 112.17 KDa which may used as markers for this genotype. The results showed that the henbane ecotype from El-Maghara has two positive bands at the molecular weights ; 190.57 and 97.00 KDa as a marker bands for this ecotype. Concerning henbane ecotype from Shalateen, it was found that four marker bands at the molecular weights ; 22 .41, 163.33, 82.06 and 76.86 KDa. On the other hand, the band number 8 at the molecular weight 171.65 appeared in the henbane ecotype from both of El-Maghara and Shalateen regions. Four common bands at the molecular weights 127.55, 95.08, 88.37 and 68.05 KDa, respectively in the henbane genotype from the three regions. 2. The obtained results three isozyme systems in henbane (Hyoscyamus muticus L.):1.A total of four peroxidase bands were obtained. Results revealed that two positive bands were characterized in the henbane ecotype from Wadi Sudr as a marker bands at Rf 0.487 and 0.633, respectively. Only one positives pecific band was characterized in the henbane ecotype from both of El-Maghara and Shalateen regions at Rf 0.165. 2.The bands of poly phenyl oxidas were clearly revealed, but polymorphism was detected only in one band at the Rf 0.449 in the henbane ecotype from Shalateen and El-Maghara regions.

Présentation étendue (EULC)

Page publiée le 18 février 2019