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Ain Shams University (2018)

MOLECULAR CHARACTERIZATION OF SOME DATE PALM GENOTYPES IN EGYPT

ABU-AFIFEH, ALI ABEDEL-HAMEED

Titre : MOLECULAR CHARACTERIZATION OF SOME DATE PALM GENOTYPES IN EGYPT

Auteur : ABU-AFIFEH,ALI ABEDEL-HAMEED.

Etablissement de soutenance : Ain Shams University

Grade : Doctor of Philosophy (PhD) in Agricultural Science (Genetics) 2018

Résumé
This study was conducted at Cairo University Research Park (CURP), during the period 2016 - 2018. Palm is one of the most important strategic crops in the Middle East and North Africa region where dry and semi-arid environments predominate. Egypt leads the list of countries producing dates in the world, where production is about 1.7 million tons annually, and the number of palm trees in Egypt about 16 million trees. Due to the importance of palm trees globally and in Egypt in specially, it is necessary to preserve, protect and document these varieties as Egyptian varieties to preserve the property rights of genetic resources. In this study, DNA fingerprinting techniques were applied to ten genotypes of seeded date palm trees, that distinct in their production, morphological and biological qualities. where each of the following techniques has been used : RAPD, ISSR, SSR, F-AFLP and BNA Barcoding In the beginning, the samples were collected from their Original home in the New Valley Governorate, DNA was then extracted from the samples and the DNA fingerprinting techniques were applied. It’s used 10 RAPD primers, 10 ISSR Primers, 8 SSR primers, 6 AFLP combined primers, and 4 specialized Barcoding genes : rbcl, matk, rpoC1, and ycf5. And by using the Bioinformatics software, 10 RAPD and 10 ISSR primers showed genetic variations among the studied seeded date palm, and 18 RAPD and 18 ISSR unique markers were identified for the studied samples, and showed genetically variations among the studied samples. Also, when SSR technique was applied, 12 SSR unique markers showed were identified the studied each studied sample. Also when F-AFLP technique, 33 AFLP unique markers showed were identified the studied each studied sample. As for the DNA Barcoding technique, the sequences were readed for each of the four bands using Next Generation Sequencing (NGS) to detect specific differences in the sequences of each studied sample. These sequences were used to record at international DNA database NCBI Gene Bank. Applied importance of the study : This study is a part of the Palm Development Program in the Arab States, which was launched and funded by the Arab Center for the Studies of Arid Zones and Dry Lands (ACSAD), which aims to develop and nurture date palm in the Arab countries. This study proved the identification of the unknown seeded date palm and recorded them in the international DNA database Gene Bank.

Présentation étendue (EULC)

Page publiée le 13 février 2019