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Ain Shams University (2017)

STUDIES ON PRODUCING POTATO SEED USING SOILLESS CULTURE

RASHWAN, MOHAMED MAHMOUD MAAMOUN.

Titre : STUDIES ON PRODUCING POTATO SEED USING SOILLESS CULTURE

Auteur : RASHWAN, MOHAMED MAHMOUD MAAMOUN.

Etablissement de soutenance : Ain Shams University

Grade : Doctor of Philosophy (PhD) in Agricultural Science (Biotechnology in Arid Land) 2017

Résumé partiel
The present study was conducted in the tissue culture laboratory (Star moon company, Giza government) for Agriculture during the period from 2013 to 2015. The aim of this study was to evaluate the efficiency of different soilless culture systems (Aeroponic, Deep water and Control) for prebasic seed potato production, taking into account the origin of propagative material seed (mini tubers and plants from in vitro culture). This work was divided into two experiments. 1- Micropropagation of potato virus-free plantlet : Potato virus-free plantlet (meristem – derived) 6-9 cm having 1-15 nodels grown in vitro (cvs, Spunta, Diamant and Hermes) A- Rapid micropropagation of plantlets in vitro plantlets were subcultural into shoots on inducing medium containing MS salts supplemented with 3% sucrose, 1 mg/L IAA and 0.04 mg/L Kin solidified with 0.7% agar. B- High quality planting material and rapid production of many uniform plants have been obtained and the 0.3 mg/L of both BA and Kin is the best concentration in the medium since it gave the highest height, of plantlet, longest root, high no. of branches or nodes and more weight of plantlet than there concentration No. clear difference between the cultivars was found (Spunta, Diamant and Hermes) C- The influence of various sucrose concentrations (0, 30, 60 and 90 gm/L) on plantlet height., root length, number of branches, number of nodes and plant weight, using three cultivars Spunta, Diamant and Hermes) were studied the data indicate that all growth plantlet height., root length, number of branches, number of nodes and plant weight) increasing of sucrose concentrations over all cultivars used. 2. Soilless culture system All shoots of the three potato cultivars which obtained from the first experiment were transferred to the rooting medium containing IBA at 1.0 mg/L. After two weeks the complete plantlets produced were washed several times and transferred from the culture tubes into plastic pots filled with peat-moss and perlite at ratio of 1:1 (v:v) and immediately transferred to green house under mist. The plantlets were fertilized with El-Behairy, 1994 solution once a week for a period of 40 days. After that the plants were cultured into soilless culture using two systems i.e., aeroponic and deep water system beside the control (sandy soil) Planting dates were 15th and 20th November in the first and second seasons respectively.

Présentation étendue (EULC)

Page publiée le 12 février 2019