Informations et ressources scientifiques
sur le développement des zones arides et semi-arides

Accueil du site → Master → Egypte → Phytochemical and Biological Study of Pelargonium peltatum (L.) L’ Her. family Geraniaceae cultivated in Egypt

Cairo University (2019)

Phytochemical and Biological Study of Pelargonium peltatum (L.) L’ Her. family Geraniaceae cultivated in Egypt

Fatma Hassan Mohammed Nossier

Titre : Phytochemical and Biological Study of Pelargonium peltatum (L.) L’ Her. family Geraniaceae cultivated in Egypt

Auteur : Fatma Hassan Mohammed Nossier

Etablissement de soutenance : Cairo University

Grade : Master in Pharmaceutical Science (Pharmacognosy) 2019

Résumé
Several herbs belong to genus Pelargonium (Arabic : عتر) are noted with considerable economic importance in ornamental plants market. Pelargonium originates from South Africa, where more than 200 species are cultivated. In Egypt, Pelargonium species are cultivated in Upper Egypt, mainly Beni Suef and Faiyum. Some Pelargonium species contain volatile oil called geranium oil which has economic value in perfume industry and cosmetics. Also, geranium oil has biological importance as it was reported to possess antibacterial, antifungal, anti-oxidant, insect repellent and other activities. Pelargonium peltatum (L.) L’Her. cv. Balcon lilla is characterized by green, succulent and palmately lobed leaves. The color of the flowers is pale pink. The preliminary phytochemical screening followed by TLC of the 70% ethanolic extract and its fractions revealed that n-hexane and methylene chloride fractions were rich in sterols and triterpenes, while ethyl acetate and n-butanol fractions were rich in flavonoids and tannins. This is the first study of the volatile components of Pelargonium peltatum (L.) L’Her. and the first time to use head space GC/MS for analyzing the oil of Pelargonium species. five compounds were identified, namely ; β-myrcene (44.86%), (-)-β-pinene (35.89%) -(-)α-pinene (10.4%), p-mentha-1(7),8-diene (5.14%) and camphene (3.71%). Four compounds were isolated and identified from the ethyl acetate fraction ; 1-O-monogalloyl-β-glucose, gallic acid, ethyl gallate and 1,2,3,4,6-pentagalloyl-β-D-glucopyranose (PGG). The total phenolic and flavonoids contents were 19.3992 mg GAE/g and 2.7203 mg QE/g of dry weight, respectively. HPLC/MS profiling revealed the presence of thirty one compounds, twelve flavonoids and nineteen phenolic acid derivatives. Seventeen compounds were identified for the first time in the Pelargonium species. It is the first HPLC profiling of Pelargonium peltatum (L.) L’Her. cv. Balcon lilla. In vivo and in vitro screening of biological activities of the 70% ethanolic extract of Pelargonium peltatum (L.) L’Her. leaves and its fractions revealed that n-hexane fraction showed the most potent analgesic activity. The 70% ethanolic extract showed analgesic, anti-inflammatory and anti-hyperglycemic activities. Methylene chloride and ethyl acetate fractions showed high anti-inflammatory and anti-oxidant activities. The most effective cytotoxic fraction on the three tested cell lines was methylene chloride fraction. The total phenolic and flavonoids contents were19.3992 mg GAE/g and 2.7203 mg QE/g of dry weight, respectively. HPLC/MS profiling revealed the presence of thirty one compounds, twelve flavonoids and nineteen phenolic acid derivatives. Seventeen compounds were identified for the first time in the Pelargonium species. It is the first HPLC profiling of Pelargonium peltatum (L.) L’Her. cv. Balcon lilla. In vivo and in vitro screening of biological activities of the 70% ethanolic extract of Pelargonium peltatum (L.) L’Her. leaves and its fractions revealed that n-hexane fraction showed the most potent analgesic activity. The 70% ethanolic extract showed analgesic, anti-inflammatory and anti-hyperglycemic activities. Methylene chloride and ethyl acetate fractions showed high anti-inflammatory and anti-oxidant activities. The most effective cytotoxic fraction on the three tested cell lines was methylene chloride fraction.

Présentation

Page publiée le 3 mars 2019