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Universidade Estadual Paulista (UNESP) 2006

Produção de xilanase por fungos filamentosos isolados de solo de área de caatinga

Simões, Maria Lúcia Garcia

Titre : Produção de xilanase por fungos filamentosos isolados de solo de área de caatinga

Auteur : Simões, Maria Lúcia Garcia

Université de soutenance : Universidade Estadual Paulista (UNESP)

Grade : Mestre em Ciências Biológicas 2006

Résumé
In this study, 67 strains of filamentous fungi were isolated from caatinga area, the collections were performed during the dry and rainy period, aiming to know the biodiversity of this not explored bioma and to evaluate the potential of these fungi to produce xylanase. Some of the strains were not identified due to the lack of specific methodologies and others were identified through microscopic and biochemical methods. A selection of the fungi which were considered potentially producers of xylanase was carried out in Voguel medium containing 1% of xylan as an only carbon source, and through a Automatized Turbidimetric Method (Bioscreen - C) the best carbon source for the fungi growth was evaluated. The results obtained in SBM were not expressive when compared to the ones obtained in WBM. The best xylanase producers in WBM were cultivated in Voguel liquid medium and WBM adding individually other carbon sources at 1% (carboxymethylcellulose -CMC ; xylan ; and the best growth carbon source determined by Bioscreen-C) at appropriated temperatures, pH 5,0 ; for 5 days, and a spore concentration of 1 x107 spores/mL. The CMC addition caused catabolic repression in xylanase production by these fungi, xylan addition showed the same effect, but Trichoderma viride 13 and Aspergillus niger 11 which have their activities increased from 39,21 U/mL to 143,0 U/mL and from 33,4 U/mL to 57,1 U/mL, respectively

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