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Islamia University of Bahawalpur (2015)

STUDIES ON CHARACTERIZATION AND DISTRIBUTION OF CULTURABLE BACTERIA IN CHOLISTAN DESERT RHIZOSPHERES

ASLAM, MUHAMMAD

Titre : STUDIES ON CHARACTERIZATION AND DISTRIBUTION OF CULTURABLE BACTERIA IN CHOLISTAN DESERT RHIZOSPHERES

Auteur : ASLAM, MUHAMMAD

Université de soutenance : Islamia University of Bahawalpur

Grade : Doctor of Philosophy (PhD) 2015

Résumé
Major aim of the study presented in this manuscript was to isolate, characterize and determine distribution of culturable bacteria in rhizospheres of Cholistan desert plants. We isolated bacteria from the rhizosphere of four Cholistan desert plants including Haloxylon salicornicum, Panicum antidotale, Lasiuarus scindicus and Leptadenia pyrotechnica. All the isolates were subjected to morphological, biochemical and molecular characterization. Total 342 bacterial isolates including 282 thermophiles and 60 mesophiles were isolated from the rhizospheric soil of Leptadenia pyrotechnica. Physical and biochemical characterization was carried out following standard protocols and procedures. Molecular analysis involved 16S rRNA profiling, RFLP and DNA sequence analysis. PCR amplicons of selected bacteria having distinct RFLP pattern were analyzed through DNA sequence analysis. Finally DNA sequences were subjected to BLAST analysis to reveal the identity of bacterial species. Results indicated that Bacillus subtilis, Brevibacillus borstelensis, and Bacillus licheniformis are present in the rhizosphere of majority of the analyzed rhizospheres. Brevibacillus borstelensis and Bacillus subtilis are the most common species present in the rhizosphere of Haloxylon salicornicum (70.0 %) and Panicum antidotale (PAJF series ; 61.10 %), respectively. However, Bacillus licheniformis was predominantly found in the rhizosphere of Panicum antidotale (PAMA series ; 45.0 %) and Lasiuarus scindicus (78.95 %). In addition to the bacillus species low frequency of some other bacterial species like Pseudomonas fluorescens (Panicum antidotale ; PAJF series), Paenibacillus dendritiformis (Panicum antidotale ; PAMA series), Stenotrophomonas rhizophila and Pseudomonas marginalis (Lasiuarus scindicus) was noticed in the studied rhizospheres. It is concluded that every plant has a unique rhizospheric bacterial community that needs to be explored to understand the factors contributing towards plant growth and health. This study is the first to investigate the rhizosphere of Cholistan desert plants. There is need to further extend the study to get an insight about the nature of the bacteria inhabiting the rhizosphere of Cholistan desert plants and exploit these bacteria for the benefit of mankind. Présentation (Pakistan Research Repository)

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