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Université Mohamed Boudiaf M’sila (2018)

Extraction, evaluation of antioxidant and antibacterial activities of Juniperus oxycedrus

BEGHRICHE, Abdenour

Titre : Extraction, evaluation of antioxidant and antibacterial activities of Juniperus oxycedrus

Auteur : BEGHRICHE, Abdenour

Université de soutenance : Université Mohamed Boudiaf M’sila

Grade : Master Académique 2018

Résumé
The aim of this study is the determination of the phytochemical constituent and evaluation of the antioxidant and antibacterial activities of Juniperus oxycedrus L. leaves extracts and essential oil. J. oxycedrus has many pharmacological and therapeutic properties including antimicrobial, and antioxidant activities. Due to this importance the leaves was analyzed for preliminary phytochemical studies. The qualitative analysis using TLC showed the presence of flavonoids in different stutied extracts. DPPH free radical scavenging method was used for antioxidant activity analysis. All the extracts exhibit a good antioxidant activity ; soxhlet ethanolic extract exhibited the highest radical scavenging activity with an IC50 values 0.25μg/ml. The extracts were also tested for their possible in vitro antibacterial activity against two bacterial strains (Escherichia coli and Staphylococcus aureus) using the disc diffusion method. The results of showed that the highest activity was observed to decoction extract (diameter of zone inhibition 13 mm) against Staphylococcus aureus and no effect was observed on E.coli, while essential oil give a positive result for both strains.

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Page publiée le 10 octobre 2019