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Islamic University – Gaza (2008)

Biomass Production of Saccharomyces cerevisiae (BAKER’S YEAST) Using the Cactus Cladodes Extract as A Culture Medium

Hammad, Majed Shaban

Titre : Biomass Production of Saccharomyces cerevisiae (BAKER’S YEAST) Using the Cactus Cladodes Extract as A Culture Medium

Auteur : Hammad, Majed Shaban

Université de soutenance : Islamic University – Gaza

Grade : MASTER DEGREE OF BIOLOGICAL SCIENCES 2008

Résumé
This study aims to evaluate Cactus opuntia cladodes (COC) extract as an alternative medium for producing Saccharomyces cerevisiae (baker’s yeast) (BY), and to optimize the environmental conditions of its growth (pH, temperature). The study design was a comparative study. COC were obtained from the boarders of a farm in Northern governate at the age of 1-2 years. The crude COC extract was diluted to 50% before using. The preactivated BY sample used also diluted to 10-7. Experiment was carried out on both surface and submerged COC extract cultures. The control media were both potatoes dextrose agar and broth (PDA, PDB). SPSS system was used to analyze the obtained data. The results showed that the BY can grow and proliferate in COC extract (50% dilution) where the average specific growth rate (µ) was 0.21 h.-1 The optimum pH for yeast growth was about (4.0) and the optimum temperature for good specific growth rate was 30 0C. The average specific growth rate of BY in PDB was 0.25 h-1 and the optimum pH and temperature were 4.0 and 30 0C, respectively. It was found also that 29.0 g/L of yeast (dry weight) was produced in COC extract, while 37.0 g/ L was produced in PDB after 72 hours growth. It was found also that the total yeast dry weight that produced in COC extract was about 41.3 g/L, while an equal quantity of PDB produced about 54.1 g/L. The study found also that 1000g fresh weight of COC can produce about 62.0 g of BY. It can be concluded that COC extract could be a good medium for producing BY under restricted economical conditions.

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Page publiée le 9 janvier 2020