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Ain Shams University (2018)

Molecular and Serological Studies on Family Chlamydiaceae in Camel and Small Ruminants in the Egyptian Desert

Allam, Sahar Ali Hussein

Titre : Molecular and Serological Studies on Family Chlamydiaceae in Camel and Small Ruminants in the Egyptian Desert

Auteur : Allam, Sahar Ali Hussein.

Etablissement de soutenance : Ain Shams University

Grade : Doctor of Philosophy (PhD) in Microbiology 2018

Résumé
This study was conducted on male and female camels ages ranged from 5-12 years from Matrouh and Marut Research station that belongs to the Desert Research Center in Al-Amria, Alexandria, In addition to ewes and goats with age 3 to 5 years. These animals had symptoms of respiratory diseases such as nasal secretions, and the secretions of the eye some were apparently healthy. Small ruminants suffered from repeated abortions and were in contact with camel in Marut. Sampling obtained in the interval between August (2014) to January (2015). Quantitative Real time PCR was carried out to investigate the presence of Chlamydiae in collected vaginal, occular, nasal and fecal swabs from randomly selected camels, ewes and she- goats. The positivity were 15.6% (n=7/45), 7.14% (4/56) and 12.8% (5/39) for the examined camels, ewes and she-goats respectively. Linear regression revealed an inverse proportion relation between Cq values and amount of the organism in the samples. The enzyme-linked immunosorbent assay (ELISA IDEXX) was carried out to detect the presence antibodies against Chlamydophila abortus in sera of randomly examined camels, ewes and she goats. Chlamydophila abortus antibodies were detected in 8.7% (n=24/276) of the tested camels with age range between 6- 12 years, all positive camels were females Prevalence of chlamydophilosis in camel was 8.3% in Al-Amria region, while its prevalence was 9.09% in Matrouh. Statistical analysis according to IBM SBSS revealed that the ELISA results in camels was significant (sig=0.00) was less than (α=0.05) with confidence degree 95%. There was a significant difference between ELISA in females than in males. No significant difference between ELISA data in Matrouh and in El-Amria. There was a significant difference between ELISA within categories of age, and so age has significant effect on the values of ELISA in camel. Enzyme assay was carried out on the serum of ewes and she-goats to predict the presence of antibodies to Chlamydophila abortus in randomly selected ewes and she-goats with experienced abortion from the desert research center in Al-Amria. The seroprevalence in ewes was 3.6% (8/224), these ewes having antibody titers ranging from 39% to 49.3%, while the seroprevalence in she-goats was %.15.4% (20/130), titers ranging from 41.481% to 52.628%. Statistical analysis was done by IBM SPSS to determine the significance of ELISA to detect Chlamydophila abortus in ewes and she-goats in addition the impact of age on the infection rate. ELISA results in ewes and she-goats were significant with confidence degree 95%. In ewes ; no significant difference between ELISA data within categories of age suggesting that the age has no significant effect on the value of ELISA data in them, although, there was a significant difference between the data in She-goats with age 3 and 4 years. All seropositive ewes and she goats were also positive for qrt-PCR. Significant correlation between IDEXX and real time PCR for goats was detected by Chi-square. However, it was noticed that some of the seropositive camels were negative in qrt-PCR which suggested acute infection. To the best of our knowledge this study is the first molecular evidence of camel chlamydophilosis in the desert of Egypt, and the first record in Egypt to detect antibodies against Chlamydophila abortus in camels with respiratory diseases or apparent healthy camels.

Présentation étendue (EULC)

Page publiée le 10 avril 2020