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Menoufiya University (2019)

STUDIES ON PEANUT BRADYRHIZOBIUM BROTH INOCULA AS AFFECTED BY IRRIGATION WATER QUALITY IN NEWLY RECLAIMED DESERT SOILS

Abd El Salam, Sameh Senosy Abd El Gawad.

Titre : STUDIES ON PEANUT BRADYRHIZOBIUM BROTH INOCULA AS AFFECTED BY IRRIGATION WATER QUALITY IN NEWLY RECLAIMED DESERT SOILS

Auteur : Abd El Salam, Sameh Senosy Abd El Gawad.

Etablissement de soutenance : Menoufiya University.

Grade : Master of Science (MS) in Agriculture Sciences 2019

Résumé
A greenhouse experiment was carried out as a pot experiment during summer season of 2016 in the Experimental Greenhouse of Department of Soil science, Faculty of Agriculture, Shbin El Kom, Menoufia University, to investigate and evaluate the efficiency of both liquid and solid inoculum of Bradyrhizobium spp (N2-fixing bacteria) alone or with plant growth promoting rhizobacteria (PGPR) ; Azotobacter chroococcum DSM 2286, as co-inoculation to inhibit the negative impact of salinity of irrigation wateron peanut plants grown in cultivated sandy soils.This soilwas collected from the surface layer (0- 30 cm) of the cultivated regions of Sadat city Menoufia Governorate, Egypt.As well as to comparatively evaluate the responses and identify the most suitable cultivar either Giza 6 or Gregoryto the studied treatments. Peanut seeds were inoculated with solid or liquid inoculum of Bradyrhizobium alone or with Azotobacter chroococcum DSM 2286 (PGPR) as co-inoculation, and planted in a sandy soils. Azotobacter chroococcum DSM 2286 originally isolated from the salt affected soil in Wadi El Natroon, Behiera Governorate, Egypt, it‘s having higher survival capabilities at different salinity levels, relatively higher potentials of nitrogenase activity, and P solubilization capacity, and IAA production.These strain was previously defined by genotypic identification which performed by amplification and partial nucleotide sequencing of the 16s rihbosomal DNA (16s rDNA) (El Zemrany et al., 2015). At 75 days after sowing, nodulation, dehydrogenase activity of rhizospheric soil of two peanut cultivars, fresh and dry weights of the growing plants and their elemental composition, mainly contents of nitrogen, phosphorus, potassium, iron, zinc and manganese were determined, seed, straw and pods weight (gm/plant), as well as quality traits of the seed of % proteinand oil%, were determined after harvest (130 days) of the two peanut cultivars. Saline irrigation water was collected from : i- BahreShibine El Kom, as a source of Nile Water (563 mg L-1) (W1), ii- Groundwater artesian well,about 1000 mg L-1 (W2) and iii- Groundwater artesian wellabout 2000 mg L-1 (W3), were collected from cultivated regions of Sadat city. The standard recommended cultivation practices had been performed in the present study.

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Page publiée le 4 avril 2020