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Shendi University (2018)

Investigation of Oils from Some Sudanese Medicinal Herbs

Ahmed, Asrar Adil Algray

Titre : Investigation of Oils from Some Sudanese Medicinal Herbs

Auteur Ahmed, Asrar Adil Algray

Université de soutenance : Shendi University

Grade : M.Sc. Degree in Chemistry 2018

The essential oils from Cymbopogon citratus, Brassica nigra and Cymbopogon nervatus were analyzed by GC-MS. In addition, these oils were investigated for antimicrobial activity. GC-MS revealed that the dominant constituents in Cymbopogon citrates oil are ; 2,6-octadienal, 3,7-dimethyl (E) (43.20%), 2,6-octadienal,3,7-dimethyl (Z) (31.36%) and 5, hepten-2-one, 6-methyl (3.87%). The major constituents in the oil of Brassica nigra were ; 13-docosenoic acid, methyl ester (35.65%), 9,12-octadecadienoic acid (Z,Z) , methyl ester (18.20%), 11-eicosenoic acid, methyl ester (12.82%), 9-Z-octadecenoic acid, methyl ester (8.90%), 9,12,15-octadecatrienoic acid, methyl ester (8.60%), hexadecanoic acid methyl ester (3.83%), 15-tetracosenoic acid, methyl ester (3.77) and Cis-11-eicosenoic acid, methyl ester (2%). Most dominant constituents in the oil of Cymbopogon nervatus are ;P-mentha-1(7),8-dien- 2-ol (24.27%), trans-p-mentha-1(7),8-dien-2-ol (21.07%), trans-p-mentha- 2,8-dienol (13.28%), carveol (10.43%), cis-p-mentha-2,8-dien-1-ol (9.43%), ]1,1-bicyclopentyl[-2-one (5.65%), (-)-carvone (5.27%) and Dlimonene (2.41%). In the disc diffusion bioassay, Cymbopogon citrates oil showed excellent antibacterial activity against all test bacteria at 100 mg/ml but did it not exhibit any anticandidal activity. It also showed excellent activity against S. aureus and P. aeruginosa at 50 and 25mg/ml. Significant antimicrobial activity was observed for Cymbopogon nervatus oil at 100 mg /ml for all test organisms. Brassica nigra oil showed excellent antimicrobial activity at 100 mg/ml against all test organisms except for S.v aureus. It also exhibited significant activity against B. subtilis and E. coli at 50 and 25 mg/


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