Informations et ressources scientifiques
sur le développement des zones arides et semi-arides

Accueil du site → Doctorat → Ouganda → Genetic resistance to adzuki bean bruchid (Callosobruchus chinensis) in soybean

Makerere University (2019)

Genetic resistance to adzuki bean bruchid (Callosobruchus chinensis) in soybean

Msiska, M.U.

Titre : Genetic resistance to adzuki bean bruchid (Callosobruchus chinensis) in soybean

Auteur : Msiska, M.U.

Université de soutenance : Makerere University

Grade : DOCTOR OF PHILOSOPHY IN PLANT BREEDING AND BIOTECHNOLOGY 2019

Résumé partiel
Soybean [Glycine max (L.) Merrill], an annual legume that belongs to the family Fabaceae is grown in every continent for its high protein (40%) and oil (20%) content. Soybean was introduced to Uganda in the 1900s. However, for the first time in Uganda, soybean is threatened by a storage pest Callosobruchus chinensis. C. chinensis causes tremendous losses because of its high fertility, ability to re-infest, short generation times and irreversible damage which is direct on the grain. C. chinensis causes overall seed weight loss, loss of seed viability and altered nutritional quality. Utilization of resistant varieties is the most effective, economical and environmentally sustainable method but it is obstructed by lack of sources of resistance and information on genetics of inheritance. Consequently, studies were undertaken to establish sources, basis and inheritance of resistance to C. chinensis in soybean. The specific objectives of the study were to :- (i) Identify sources of resistance to C. chinensis in the available germplasm in Uganda, (ii) determine the biochemicals associated with C. chinensis resistance in soybean and (iii) determine the mode of inheritance of resistance to C. chinensis in soybean. The studies were carried out at Makerere University Agricultural Research Institute–Kabanyolo (MUARIK) and National Crops Resources Research Institute (NaCCRI)- Namulonge between 2015 and 2018. Four hundred and ninety eight genotypes from Uganda, Zimbabwe, USA and Taiwan were infested with 1-3 day old unsexed bruchids under a no choice test in a randomized complete block design with three replicates. Genotypes showed variations in response to C. chinensis indicating differences in resistance levels implying that they contain different amounts of intrinsic and extrinsic factors responsible for resistance. The highest resistance was observed in genotypes AVRDC G8527 and PI G89 while AVRDC G 2043 was the most susceptible. Therefore, AVRDC G8527 and PI G89 were the identified sources of resistance. Based on the results of the no choice test, eight genotypes with varying resistance levels were assessed for biochemical concentrations. The biochemical concentrations of soybean indicated that high tannins, total antioxidants, peroxidase activity and low flavonoids were associated with resistance to C. chinensis. The study established that secondary and not primary metabolites were associated with resistance to C. chinensis in soybean.

Présentation

Version intégrale

Page publiée le 13 mai 2020