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Universidade do Minho (2015)

RNA-seq analysis of the Quercus suber root response to drought

Análise de RNAseq da resposta à secura de raiz de Quercus suber

Titre : RNA-seq analysis of the Quercus suber root response to drought

Análise de RNAseq da resposta à secura de raiz de Quercus suber

Auteur : Magalhães, Alexandre Miguel Papadopoulos

Université de soutenance : Universidade do Minho

Grade : Mestrado em Plant Molecular Biology, Biotechnology and Bioentrepreneurship 2015

Résumé partiel
Cork oak (Quercus suber L.) is a typical species from the Portuguese Mediterranean forest, usually growing in dry environments. Mainly due to its high economic value, cork oak has been considered a protected plant species and a national species of interest. Factors resulting from climate changes, e.g. increased temperature, high light intensity, drought and air pollution are directly affecting the development of plants and reducing their productivity. Next generation sequencing, in particular RNAseq, has proven to be a powerful tool with a diverse range of applications, and provides fundamental insight into the structure and regulatory aspects of gene networks, particularly when organisms without avalible OMICs resources, such as a sequence genome, are studied. To address the cork oak’s response and regulation to water availability, differential transcriptomic analysis was performed in roots of young plantlets subjected to moderate and high drought conditions, comparing to well-irrigated plants. Photosynthetic activity measured by PAM fluorometry and photosynthetic pigment content were used as physiological indicators of plant fitness. cDNA was obtained from previously droughtstressed roots RNA, and sequencing was carried out by Roche’s 454 technology resulting in three libraries, of a 1.8 million reads in total, later assembled into 21012 unigenes, that would be used for mapping steps, in order to identify differential expression.

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