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Universidade de Tras os Montes E Alto Douro (2018)

Effectiveness of Ascorbic Acid and Zinc as drought antagonists in bread wheat plants monitored by different DNA markers

Alves, João Tiago Roque

Titre : Effectiveness of Ascorbic Acid and Zinc as drought antagonists in bread wheat plants monitored by different DNA markers

Auteur : Alves, João Tiago Roque

Université de soutenance : Universidade de Tras os Montes E Alto Douro

Grade : Mestrado 2018

Résumé partiel
Bread wheat is economical important worldwide and due to the climate changes drought is frequent and it is affecting the yield and quality of this crop. Ascorbic Acid (AsA) and Zinc (Zn) can act as drought antagonists minimizing the negative effects of this abiotic stress. Drought and other abiotic stresses generate genomic instability in plants that can be monitored by molecular markers. Conserved DNA Domain Polymorphism (CDDP) and Start Codon Targeted (SCoT) are gene-targeted markers that are able to assess variation in functional genomic regions ; retrotransposons (RTNs)-based markers may moniter the RTNs activity and Random Amplified Polymorphic DNA (RAPD) are able to screen genomic instabilities throughout the genome. In this study, we intend to test the effectiveness of seed priming and/or foliar application with Ascorbic Acid (AsA) or Zinc sulphate heptahydrate (ZnSO4.7H2O) for drought mitigation in bread wheat plants of cv. „Jordão‟ by evaluating the genomic instability in treated plants relative to control plants both under two water regimes (watering and drought) using CDDPs, SCoTs, Inter-Retrotransposon Amplified Polymorphism (IRAP), Retrotransposon-Microsatellite Amplified Polymorphism (REMAP), inter-Priming Binding Site (iPBS), Inter-Simple Sequence Repeats (ISSRs) and RAPDs. The genomic instability was reflected by the appearance of new bands and loss of bands in treated plants relative to control

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