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University of Mohaghegh Ardabili (2018)

Assessment of genetic variation in Caucasian whortleberry (Vaccinium arctostaphylos L.) of Ardabil province by molecular markers

Puor Mohabat, adel

Titre : Assessment of genetic variation in Caucasian whortleberry (Vaccinium arctostaphylos L.) of Ardabil province by molecular markers

Auteur : Puor Mohabat, adel

Université de soutenance : University of Mohaghegh Ardabili

Grade : Master 2018

Résumé
Caucasian whortleberry (Vaccinium arctostaphylos L.) is perennial plant from Ericaceae family, that fruit and leaves this plant were used to treat diabetes and blood pressure in Iran traditional medicine. This species is the only Vaccinium species in Iran and grows under the shade of beech trees. The young leaves of 30 qaraqat genotypes were collected from three different regions in its natural habitat in Ardebil province, including : Souha, Saghezchi andzandaneh on Khalkhal-Punel Road. Total genomic DNA of genotypes was extracted accord¬ing to the CTAB method with a few changes. Six ISSR primers generated 30 scorable bands that 19 were polymorphic, with average polymorphism information content (PIC) of 0.34. The genetic similarity of the genotypes with Jaccard’s coefficient shows the lowest genetic distance between 7-Souha and 1-Zandan genotypes and the highest genetic distance between 10-Souha and 5- Saghezgenotypes. In the Neighbor-Joining dendrogram the genotypes clustered in to five major groups based on the Jaccard’s coefficient. The study of the samples was also performed using eight SCoT markers. The highest number of bands multiplied by SCoT1 marker. Average polymorphic information content was 0.31 and the highest polymorphism was calculated to SCoT5 marker with 0.45. The genetic similarity of the samples with Jaccard’s coefficient varied from 0.15 to 0.82, with the lowest genetic distance between 6-Souha genotypes with 8-souha and the highest genetic distance between genotypes 10-Souha and 5- Saghez was observed. Drawing the dendrogram using the NJ clustering algorithm method, genotypes were divided into six groups.

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