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University of Mohaghegh Ardabili (2017)

Documenting biodiversity in some native Salvia species of Iran using rbcL barcode and evaluation of phytochemical diversity in Salvia spinosa and Salvia

Alibabaei, Fariba

Titre : Documenting biodiversity in some native Salvia species of Iran using rbcL barcode and evaluation of phytochemical diversity in Salvia spinosa and Salvia

مستندسازی تنوع زیستی برخی از گونه¬های سالویا بومی ایران با استفاده از بارکد rbcL و ارزیابی تنوع فیتوشیمیایی دو گونه¬¬ی Salvia spinosa و Salvia

Auteur : Alibabaei, Fariba

Université de soutenance : University of Mohaghegh Ardabili

Grade : Master 2017

Résumé
Salvia L. is an important genus from Lamiaceae family that has about 900 species in the world, including 58 species in Iran, Some of these are annual and perennial herbaceous, which 17 species of them are endemic. In this study, Salvia nemorosa and Salvia spinosa were studied in terms of essential oil content and variety of chemical compounds were present in them. The essential oil samples was extracted by steam distillation and their chemical compounds were detected by Gas Chromatography–Mass Spectrometry (GC-MS). The major components of essential oil of Salvia nemorosa collected from Arasbaran-Varzaqan region inclide α-Thujone (32.66%), 1,8-Cineole (22.18%) and Heptan-2-one (10.66% ), from Tabriz-Varzaqan region were Trans-Caryophyllene (17.82%), 1,8-Cineole (8.86%) and α-Cadinol (8.02%), Ardabil-Hir region contain Trans-Caryophyllene (14.33%) and 1,8-Cineole (13.67%), Ardabil-Kosar region were Trans-Caryophyllene (18.03%) and 1,8-Cineole (11.89%) and Qazvin region inclide Delta-Cadinene (8.87%), Cadinol (8.03%), Torreyol (7.94%) and trans-Caryophyllene (6.96%). The major components of essential oil of Salvia spinosa collected from the Mianeh region (route of Allahlu to Ahmadabad) were Beta-Caryophyllene (17.27%), Germacrene-D (10.82%), Benzoic acid (9.51% ) and Alpha-copaene (7.95%), Mianeh to Zanjan region contain trans-Caryophyllene (32.17%), Germacrene-D (31.23%) and Alpha-copaene (13.72%), Tabriz-Spiran village region include trans-Caryophyllene (17.42%), Germacrene-D (16.57%), Hexahydro-azocinone (11.57%), Mianeh- Ahmadabad Garoos village region contain trans-Caryophyllene (10.37%) and 1-Decene (10.14%). Also, in this study, the DNA barcoding of several native Salvia species using rbcL intergenic spacer region. The genomic DNA of all samples was extracted and PCR products were sent to Tekupozit company for sequencing. DNA Star and MEGA6 softwares were used for data analysis. The length of amplified rbcL barcodes were 600 bp, which amplifing and sequencing of them were 100% successful. The sequences analysis showed that PCR products were part of rbcL locus and belonged to Salvia genus. Using the MEGA6 software, phylogenetic trees constructed based on Neighbor-joining method with 1000 bootstraps for sequencing samples of different Salvia species as well as various genus of Lamiaceae family retrieved from NCBI database. According to the results, the rbcL barcode was not able to separate the different species of Salvia genus due to the low speed of evolution, while separated correctly different genus of Lamiaceae family from each other.

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Page publiée le 24 avril 2020