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Istituto Agronomico Mediterraneo di Bari (IAMB) 2017

Isolation and molecular characterization of X. fastidiosa from different hosts present in Apulia region (Italy)

Stancic, Marko

Titre : Isolation and molecular characterization of X. fastidiosa from different hosts present in Apulia region (Italy)

Auteur : Stancic, Marko

Etablissement de soutenance : Istituto Agronomico Mediterraneo di Bari (IAMB) - Centre international de hautes études agronomiques méditerranéennes (CIHEAM)

Grade : Master of science : Integrated pest management of Mediterranean fruit crops 2017

Résumé
In 2013 a severe disease induced by Xyllella fastidiosa caused huge damages in the olive orchards of Apulia region, South Italy. CoDiRO strain, belonging to subspecies pauca, was found to be the only agent of the infection, and 30 plant species were confirmed to be susceptible to this pathogen. Unlike Italy, in France 2 different subspecies, comprising several strains, were detected. Olea europaea is commonly used for the pathogen isolation from plant tissues. The aim of this study was to identify suitable host species, besides the olive, for the bacterial isolation, in terms of growth time and culture purity, using different approaches. Isolations were carried out from 29 different host species which were found positive by ELISA and PCR tests. Samples were collected throughout the year, once or twice per month. Pure cultures were obtained from : Olea europaea, Polygala myrtifolia, Nerium oleander and Lavandula angustifolia . For most host plant species spring and early summer proved to be the best seasons for X. fastidiosa isolation. Subsequently, Multilocus Sequence typing (MLST) performed from the obtained isolates of Lavandula angustifola and Polygala mytrifolia confirmed that all sequences have the same profile as CoDiRO strain, with no variation.

Mots clés : APULIA ; AXENIC CULTURE ; BACTERIA ; SPECIES ; XYLELLA FASTIDIOSA

Présentation (IAMB Library)

Page publiée le 13 mai 2020